Eady E A, Ross J I, Cove J H, Holland K T, Cunliffe W J
Department of Microbiology, University of Leeds, UK.
J Antimicrob Chemother. 1989 Apr;23(4):493-502. doi: 10.1093/jac/23.4.493.
Erythromycin-resistant propionibacteria isolated from the skin of antibiotic-treated acne patients were found to express four different patterns of resistance to a set of eight MLS antibiotics. The majority of isolates (48/89 strains) were constitutively resistant to 14- and 16-membered ring macrolide, lincosamide and streptogramin B-type antibiotics. MICs of josamycin (0.5-16 mg/l) and spiramycin (0.5-128 mg/l) were lower than those of erythromycin, oleandomycin and tylosin (64 to less than 512 mg/l). Two strains of Propionibacterium granulosum exhibited inducible MLS resistance. Both 14- and 16-membered ring macrolides and virginiamycin S induced clindamycin resistance in these strains. Fifteen isolates demonstrated a similar phenotype to the inducible strains but were non-inducible by erythromycin. A fourth group of strains demonstrated high level resistance to all five macrolides tested (MIC greater than or equal to 128 mg/l) but were sensitive to virginiamycin S. The phenotype displayed by these strains is not compatible with the expression of methylase genes as currently known, nor with the action of an erythromycin esterase which hydrolyses only 14-membered ring macrolides. The resistance patterns of 12 isolates could not be classified into any of the above phenotypic classes. Therefore, the majority of erythromycin resistant propionibacteria express MLS resistance which is phenotypically similar to that coded for by several well characterized RNA methylase (erm) genes.
从接受过抗生素治疗的痤疮患者皮肤中分离出的耐红霉素丙酸杆菌，对一组8种MLS抗生素表现出四种不同的耐药模式。大多数分离株（48/89株）对14元环和16元环大环内酯类、林可酰胺类及链阳菌素B型抗生素呈组成型耐药。交沙霉素（0.5 - 16mg/L）和螺旋霉素（0.5 - 128mg/L）的最低抑菌浓度低于红霉素、竹桃霉素和泰乐菌素（64至小于512mg/L）。两株颗粒丙酸杆菌表现出诱导型MLS耐药。14元环和16元环大环内酯类以及维吉尼亚霉素S均可诱导这些菌株对克林霉素产生耐药。15株分离株表现出与诱导型菌株相似的表型，但对红霉素不具诱导性。第四组菌株对所测试的所有5种大环内酯类均表现出高水平耐药（最低抑菌浓度大于或等于128mg/L），但对维吉尼亚霉素S敏感。这些菌株所表现出的表型与目前已知的甲基化酶基因表达不相符，也与仅水解14元环大环内酯类的红霉素酯酶的作用不相符。12株分离株的耐药模式无法归入上述任何一种表型类别。因此，大多数耐红霉素丙酸杆菌表现出的MLS耐药在表型上与几个特征明确的RNA甲基化酶（erm）基因所编码的耐药相似。
