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基于增强型绿色荧光蛋白（eGFP）表达，使用三重DNA分子信标检测癌细胞。

Detection of cancer cells using triplex DNA molecular beacons based on expression of enhanced green fluorescent protein (eGFP).

作者信息

Xi Dongmei, Wang Xindong, Ai Shiyun, Zhang Shusheng

机构信息

Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.

出版信息

Chem Commun (Camb). 2014 Aug 28;50(67):9547-9. doi: 10.1039/c4cc03925d.

DOI:10.1039/c4cc03925d
PMID:25012879
Abstract

A novel strategy was proposed for Ramos cell detection by combining the expression of enhanced green fluorescent protein (eGFP) with the cell aptamer recognition and the triplex molecular beacons. This system was successfully applied to cancer cell detection with high sensitivity and specificity.

摘要

提出了一种通过将增强型绿色荧光蛋白（eGFP）的表达与细胞适配体识别及三联体分子信标相结合来检测拉莫斯细胞的新策略。该系统已成功应用于癌细胞检测，具有高灵敏度和特异性。

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