Embrapa Roraima, Rodovia BR 174 km 08, Boa Vista, Roraima 69301-970, Brazil.
Laboratory of Microbiology, Department of Biochemistry and Microbiology (WE10), Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3395-3401. doi: 10.1099/ijs.0.063727-0. Epub 2014 Jul 10.
Root-nodule bacteria were isolated from Inga laurina (Sw.) Willd. growing in the Cerrado Amazon region, State of Roraima, Brazil. The 16S rRNA gene sequences of six strains (BR 10250(T), BR 10248, BR 10249, BR 10251, BR 10252 and BR 10253) showed low similarities with currently described species of the genus Bradyrhizobium. Phylogenetic analyses of sequences of five housekeeping genes (dnaK, glnII, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EK05(T) to be the closest type strain (97.4% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with the major components C16:0 and summed feature 8 (C18:1ω6c/C18:1ω7c)], the slow growth rate and carbon compound utilization patterns supported the assignment of our strains to the genus Bradyrhizobium. Results from DNA-DNA hybridizations and physiological traits differentiated our strains from the closest related species of the genus Bradyrhizobium with validly published names. Sequences of symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped together with those of B. iriomotense EK05(T) and Bradyrhizobium sp. strains BR 6610 (used as a commercial inoculant for Inga marginata in Brazil) and TUXTLAS-10 (previously observed in Central America). Based on these data, the six strains represent a novel species, for which the name Bradyrhizobium ingae sp. nov. is proposed. The type strain is BR 10250(T) ( = HAMBI 3600(T)).
从巴西罗赖马州塞拉多亚马逊地区生长的 Inga laurina (Sw.) Willd. 中分离到根瘤菌。6 株菌（BR 10250(T)、BR 10248、BR 10249、BR 10251、BR 10252 和 BR 10253）的 16S rRNA 基因序列与目前描述的慢生根瘤菌属物种相似度较低。5 个看家基因（dnaK、glnII、gyrB、recA 和 rpoB）序列的系统发育分析表明，Bradyrhizobium iriomotense EK05(T)是最接近的模式菌株（序列相似度为 97.4%或更低）。化学分类学数据，包括脂肪酸图谱（主要成分 C16:0 和特征 8 总和（C18:1ω6c/C18:1ω7c））、缓慢的生长速度和碳化合物利用模式，支持将我们的菌株分配到慢生根瘤菌属。DNA-DNA 杂交和生理特性的结果将我们的菌株与具有有效发表名称的该属最密切相关的物种区分开来。共生相关基因 nodC 和 nifH 的序列与 Bradyrhizobium iriomotense EK05(T)和 Bradyrhizobium sp. 菌株 BR 6610（巴西用于 Inga marginata 的商业接种剂）和 TUXTLAS-10（以前在中美洲观察到）的序列聚在一起。基于这些数据，这 6 株菌代表一个新的物种，提议将其命名为 Bradyrhizobium ingae sp. nov.。模式菌株是 BR 10250(T)（= HAMBI 3600(T)）。
