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来自亚马逊雨林的有效共生体马瑙斯慢生根瘤菌菌株BR 3351的基因组序列草图

Draft genome sequence of Bradyrhizobium manausense strain BR 3351, an effective symbiont isolated from Amazon rainforest.

作者信息

Simões-Araújo Jean Luiz, Rumjanek Norma Gouvêa, Xavier Gustavo Ribeiro, Zilli Jerri Édson

机构信息

Embrapa Agrobiologia, Seropédica, RJ, Brazil.

Embrapa Agrobiologia, Seropédica, RJ, Brazil.

出版信息

Braz J Microbiol. 2017 Oct-Dec;48(4):610-611. doi: 10.1016/j.bjm.2016.10.025. Epub 2017 Feb 9.

Abstract

The strain BR 3351 (Bradyrhizobium manausense) was obtained from nodules of cowpea (Vigna unguiculata L. Walp) growing in soil collected from Amazon rainforest. Furthermore, it was observed that the strain has high capacity to fix nitrogen symbiotically in symbioses with cowpea. We report here the draft genome sequence of strain BR 3351. The information presented will be important for comparative analysis of nodulation and nitrogen fixation for diazotrophic bacteria. A draft genome with 9,145,311bp and 62.9% of GC content was assembled in 127 scaffolds using 100bp pair-end Illumina MiSeq system. The RAST annotation identified 8603 coding sequences, 51 RNAs genes, classified in 504 subsystems.

摘要

菌株BR 3351(马瑙斯慢生根瘤菌)从生长在采自亚马逊雨林土壤中的豇豆(Vigna unguiculata L. Walp)根瘤中分离得到。此外,观察到该菌株在与豇豆共生时具有很高的共生固氮能力。我们在此报告菌株BR 3351的基因组草图序列。所呈现的信息对于固氮细菌结瘤和固氮的比较分析具有重要意义。使用100bp双端Illumina MiSeq系统在127个支架中组装了一个9,145,311bp且GC含量为62.9%的基因组草图。RAST注释鉴定出8603个编码序列、51个RNA基因,分为504个亚系统。

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