Uduak Okon Akpan, Ani Elemi John, Etoh Emmauel Columba Inyang, Macstephen Adienbo Ologbagno
Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo, Akwa Ibom, Nigeria.
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Cross River, Nigeria.
Niger Med J. 2014 May;55(3):230-4. doi: 10.4103/0300-1652.132049.
There are previous independent reports on the anti-thyroid property of Citrus sinensis. This isoflavones and phenolic acid-rich natural agent is widely consumed as dietary supplement, thus the need to investigate its comparative effect with a standard anti-thyroid drug on T4, T3 and thyroid stimulating hormone (TSH) levels.
To compare the effect of Citrus sinensis and carbimazole (CARB) on blood levels of thyroid hormones (T4 and T3) and TSH.
Male wistar albino rats weighing 100-150 g were employed in this research. The rats were randomly assigned to four groups of seven rats per group. Group I served as control and were administered distilled water while groups II-IV were administered with 1500 mg/kg of Citrus sinensis (fresh orange juice; FOJ), 0.1 μg/g of levothyroxine (LVT) and 0.01 mg/g of CARB, respectively, per oral once daily for 28 days. The animals were sacrificed under chloroform anaesthesia and blood sample collected by cardiac puncture and processed by standard method to obtain serum. TSH, T4 and T3 were assayed with the serum using ARIA II automated radioimmunoassay instrument.
The results showed that TSH level was significantly (P < 0.05) decreased in LVT treated group compared with the FOJ group. T4 was significantly (P < 0.05) decreased in the FOJ and CARB groups compared with the control and LVT groups. LVT significantly increased T4 when compared with FOJ group. T3 was significantly (P < 0.05) decreased in the CARB group compared with the control.
These findings suggest that FOJ alters thyroid hormones metabolism to reduce their serum levels with a compensatory elevations of TSH level in a direction similar to CARB.
先前有关于橙子抗甲状腺特性的独立报道。这种富含异黄酮和酚酸的天然物质作为膳食补充剂被广泛食用，因此有必要研究其与标准抗甲状腺药物对甲状腺素（T4）、三碘甲状腺原氨酸（T3）和促甲状腺激素（TSH）水平的比较效果。
比较橙子和卡比马唑（CARB）对甲状腺激素（T4和T3）及TSH血液水平的影响。
本研究选用体重100 - 150克的雄性Wistar白化大鼠。将大鼠随机分为四组，每组七只。第一组作为对照组，给予蒸馏水，而第二至四组分别给予1500毫克/千克的橙子（鲜橙汁；FOJ）、0.1微克/克的左甲状腺素（LVT）和0.01毫克/克的CARB，每日经口给药一次，持续28天。在氯仿麻醉下处死动物，通过心脏穿刺采集血样，并采用标准方法处理以获得血清。使用ARIA II自动放射免疫分析仪测定血清中的TSH、T4和T3。
结果显示，与FOJ组相比，LVT治疗组的TSH水平显著降低（P < 0.05）。与对照组和LVT组相比，FOJ组和CARB组的T4显著降低（P < 0.05）。与FOJ组相比，LVT显著升高了T4。与对照组相比，CARB组的T3显著降低（P < 0.05）。
这些发现表明，FOJ改变甲状腺激素代谢，降低其血清水平，并使TSH水平出现代偿性升高，其作用方向与CARB相似。
