Soto-Hernandez J L, Holtsclaw-Berk S, Harvill L M, Berk S L
Medical Service, Veterans Administration Medical Center, Johnson City, Tennessee 37614.
J Clin Microbiol. 1989 May;27(5):903-8. doi: 10.1128/jcm.27.5.903-908.1989.
Isolates of Branhamella catarrhalis from 13 patients with pneumonia, 6 patients with tracheobronchitis, and 8 patients who were colonized with the organism were studied with respect to susceptibility to the bactericidal action of normal human serum (NHS), glass slide hemagglutination (HA) of group O human erythrocytes, beta-lactamase production, and susceptibility to selected antimicrobial agents and laboratory drugs. A total of 18 of 27 isolates were serum resistant, 22 of 27 produced HA, and 21 of 27 were beta-lactamase positive. Statistically significant correlations were found between susceptibility to NHS and susceptibility to trypsin (r = +0.47; P = 0.01) and between susceptibility to NHS and HA (r = -0.48; P = 0.009). Significant correlations were also observed among several pairs of antimicrobial drugs. Analysis of variance showed that mean ampicillin MICs correlated with isolate group (r = -0.49; P = 0.03) in that the pneumonia isolates had higher MICs. Some phenotypic characteristics appeared to be independent of each other. These data suggest that important differences exist among clinically significant B. catarrhalis strains and that these differences may be due to differences in the cell wall envelope of the organism.
对从13例肺炎患者、6例气管支气管炎患者以及8例该菌定植患者中分离出的卡他布兰汉菌进行了研究，内容包括对正常人血清（NHS）杀菌作用的敏感性、O型人红细胞的玻片血凝（HA）、β-内酰胺酶的产生以及对所选抗菌药物和实验室药物的敏感性。27株分离菌中共有18株对血清耐药，27株中有22株产生HA，27株中有21株β-内酰胺酶阳性。在对NHS的敏感性和对胰蛋白酶的敏感性之间（r = +0.47；P = 0.01）以及对NHS的敏感性和HA之间（r = -0.48；P = 0.009）发现了具有统计学意义的相关性。在几对抗菌药物之间也观察到了显著相关性。方差分析表明，平均氨苄西林MIC与分离菌群相关（r = -0.49；P = 0.03），因为肺炎分离株的MIC较高。一些表型特征似乎相互独立。这些数据表明，临床上有意义的卡他布兰汉菌菌株之间存在重要差异，且这些差异可能是由于该菌细胞壁包膜的差异所致。
