Christensen J J, Gadeberg O, Bruun B
Acta Pathol Microbiol Immunol Scand B. 1986 Apr;94(2):89-95. doi: 10.1111/j.1699-0463.1986.tb03025.x.
A three-month survey revealed 29 patients at our hospital with symptoms of acute pulmonary infection, from whom Branhamella catarrhalis was isolated from lower respiratory tract specimens, in 18 cases in pure culture. Approximately 2% of all respiratory tract specimens examined during the period yielded growth of B. catarrhalis. All except one patient suffered from chronic pulmonary disease, notably chronic bronchitis. A phenotypic comparison was made between 55 strains of B. catarrhalis, of which 50 were recent isolates from lower respiratory tract specimens, and 23 Neisseria strains representing Neisseria meningitidis, Neisseria gonorrhoeae, Neisseria cinerea, Neisseria flavescens, Neisseria mucosa, Neisseria pharyngis, and Neisseria lactamica. The morphology of B. catarrhalis colonies is very characteristic, and when the diagnosis is suspected, testing for the ability to hydrolyze tributyrin may confirm it within hours. Ability to produce deoxyribonuclease is another property which differentiates B. catarrhalis from the Neisseria species. Otherwise, the combination of nitrate reduction and failure to produce acid from glucose, maltose, and sucrose establishes the diagnosis.
一项为期三个月的调查显示，我院有29例患者出现急性肺部感染症状，从其下呼吸道标本中分离出卡他布兰汉菌，其中18例为纯培养。在此期间检查的所有呼吸道标本中，约2%培养出卡他布兰汉菌。除1例患者外，所有患者均患有慢性肺部疾病，尤其是慢性支气管炎。对55株卡他布兰汉菌进行了表型比较，其中50株是近期从下呼吸道标本中分离得到的，还对代表脑膜炎奈瑟菌、淋病奈瑟菌、灰色奈瑟菌、微黄奈瑟菌、黏膜奈瑟菌、咽奈瑟菌和乳屑奈瑟菌的23株奈瑟菌进行了比较。卡他布兰汉菌菌落的形态非常有特征，当怀疑诊断时，检测其水解三丁酸甘油酯的能力可在数小时内得到证实。产生脱氧核糖核酸酶的能力是卡他布兰汉菌与奈瑟菌属鉴别的另一特性。此外，硝酸盐还原以及不能利用葡萄糖、麦芽糖和蔗糖产酸也有助于诊断。
