一种用于促卵泡激素的增强型化学发光酶免疫测定法。

An enhanced chemiluminescent enzyme immunoassay for follicle stimulating hormone.

作者信息

Sankolli G M, Lynch S S, Rudd B T, Thorpe G H, Stott R A, Kricka L J

机构信息

Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, UK.

出版信息

J Immunoassay. 1989;10(2-3):207-19. doi: 10.1080/01971528908053237.

DOI:10.1080/01971528908053237

PMID:2501358

Abstract

An enhanced chemiluminescent enzyme immunoassay for serum follicle stimulating hormone is described which involves sequential reaction of anti-follicle stimulating hormone antibody immobilised to the inside surface of an opaque microtitre plate with sample, monoclonal anti-alpha thyroid stimulating hormone antibody, and an anti-mouse IgG - horseradish peroxidase conjungate. Bound peroxidase activity was measured using a p-hydroxycinnamic acid enhanced chemiluminescent luminol-hydrogen peroxide reaction. The assay was sensitive (detection limit 0.01 mU/well) precise (intra-assay precision 2.5-8.1%, inter-assay precision 6.7-11.9%) and results obtained with this assay and a competitive radioimmunoassay were in good agreement (correlation coefficient 0.98).

摘要

本文描述了一种用于血清促卵泡激素的增强化学发光酶免疫测定法，该方法包括将固定在不透明微量滴定板内表面的抗促卵泡激素抗体与样品、单克隆抗α促甲状腺激素抗体以及抗小鼠IgG-辣根过氧化物酶缀合物进行顺序反应。使用对羟基肉桂酸增强的化学发光鲁米诺-过氧化氢反应来测量结合的过氧化物酶活性。该测定法灵敏（检测限为0.01 mU/孔）、精确（批内精密度为2.5-8.1%，批间精密度为6.7-11.9%），并且用该测定法和竞争性放射免疫测定法获得的结果具有良好的一致性（相关系数为0.98）。