Division of Marine Technology, Tokyo University of Marine Science and Technology, 2-1-6 Etchujima Koto-ku, Tokyo 135-8533, Japan.
Division of Marine Technology, Tokyo University of Marine Science and Technology, 2-1-6 Etchujima Koto-ku, Tokyo 135-8533, Japan.
Bioelectrochemistry. 2015 Feb;101:1-7. doi: 10.1016/j.bioelechem.2014.06.007. Epub 2014 Jun 24.
A novel glucose biosensor with an immobilized mediator was studied using electrochemical impedance spectroscopy (EIS) and amperometry measurements. The biosensor has a characteristic ultrathin form and is composed of a self-assembled monolayer anchoring glucose oxidase (GOx) covered with Langmuir-Blodgett (LB) films of Prussian blue (PB). The immobilized PB in the LB films acts as a mediator and enables the biosensor to work under a low potential (0.0V vs. Ag/AgCl). In the EIS measurements, a dramatic decrease in charge transfer resistance (Rct) was observed with sequential addition of glucose, which can be attributed to enzymatic activity. The linearity of the biosensor response was observed by the variation of the sensor response (1/Rct) as a function of glucose concentration in the range 0 to 25mM. The sensor also showed linear amperometric response below 130mM glucose. The organic-inorganic system of GOx and PB nanoclusters demonstrated bifunctional sensing action, both amperometry and EIS modes, as well as long sensing stability for 4 days.
采用电化学阻抗谱（EIS）和安培测量法研究了一种新型的固定化介体葡萄糖生物传感器。该生物传感器具有独特的超薄形式，由自组装单分子层固定葡萄糖氧化酶（GOx）和普鲁士蓝（PB）的Langmuir-Blodgett（LB）膜组成。固定在 LB 膜中的 PB 作为介体，使生物传感器能够在低电位（0.0V 对 Ag/AgCl）下工作。在 EIS 测量中，随着葡萄糖的连续加入，观察到电荷转移电阻（Rct）的急剧下降，这可以归因于酶的活性。通过传感器响应（1/Rct）随葡萄糖浓度在 0 至 25mM 范围内的变化来观察生物传感器响应的线性。该传感器在低于 130mM 葡萄糖时也表现出线性的安培响应。GOx 和 PB 纳米簇的有机-无机体系表现出双功能传感作用，即安培和 EIS 两种模式，以及长达 4 天的传感稳定性。
