Lenhardt Lea, Zeković Ivana, Dramićanin Tatjana, Dramićanin Miroslav D, Bro Rasmus
University of Belgrade, Vinča Institute of Nuclear Sciences, PO Box 522, 11001 Belgrade, Serbia.
Appl Spectrosc. 2014;68(5):557-63. doi: 10.1366/13-07325.
Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among honey groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication.
采用正面同步荧光光谱结合化学计量学方法，根据蜂蜜的植物来源对蜂蜜样本进行分类。在240 - 500 nm激发范围内，以30 - 300 nm的同步波长间隔，采集了三种单花蜂蜜（椴树蜜、向日葵蜜和刺槐蜜）、多花蜂蜜（草地混合蜜）以及假蜂蜜（假刺槐蜜和假椴树蜜）类型的109个样本的同步荧光光谱。对收集到的数据进行化学计量学分析，包括主成分分析和偏最小二乘判别分析。仅针对单花蜂蜜样本的模型和同时包括单花蜂蜜与多花蜂蜜组的模型，平均交叉验证分类误差分别为0.2%和4.8%。结果表明，不同蜂蜜的单同步荧光光谱因其独特的物理和化学特性而存在显著差异，并为蜂蜜组之间的清晰区分提供了充分的数据。假蜂蜜样本的光谱与真蜂蜜的光谱有明显差异，基于其同步荧光光谱很容易识别这些样本。该研究表明，这种方法是一种用于蜂蜜鉴别的有价值且有前景的技术。
