Medical University of Warsaw, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry ul. Banacha 1, 02-097 Warsaw, Poland.
Appl Spectrosc. 2014;68(6):663-71. doi: 10.1366/13-07264.
Three types of human odontogenic tumors histologically classified as compound composite odontoma, ossifying fibroma, and Pindborg tumor were characterized using mid-infrared spectroscopy (mid-IR) and solid-state nuclear magnetic resonance (ssNMR). For comparison, human jawbone and dental mineralized tissues such as dentin, enamel, and dental cement were also characterized. The studies focused on the structural properties and chemical composition of pathological tissues versus histochemically related tissues. All analyzed tumors were composed of organic and mineral parts and water. Apatite was found to be the main constituent of the mineral part. Various components (water, structural hydroxyl groups, carbonate ions (CO(3)(2-)), and hydrogen phosphate ions (HPO(4)(2-))) and physicochemical parameters (index of apatite maturity and crystallinity) were examined. The highest organic/mineral ratio was observed in fibrocementoma, a finding that can be explained by the fibrous character of the tumor. The lowest relative HPO(4)(2-) content was found in odontoma. This tumor is characterized by the highest mineral crystallinity index and content of structural hydroxyl groups. The Pindborg tumor mineral portion was found to be poorly crystalline and rich in HPO(4)(2-). The relative CO(3)(2-) content was similar in all samples studied. The results of spectroscopic studies of odontogenic tumors were consistent with the standard histochemical analysis. It was shown that the various techniques of ssNMR and elaborate analysis of the mid-IR spectra, applied together, provide valuable information about calcified benign odontogenic tumors.
三种组织学上分类为复合型混合性牙瘤、骨化性纤维瘤和 Pindborg 瘤的人牙源性肿瘤，采用中红外光谱（mid-IR）和固态核磁共振（ssNMR）进行了特征描述。为了进行比较，还对人颌骨和牙本质、釉质和牙骨质等矿化组织进行了特征描述。研究重点是病理性组织与组织化学相关组织的结构特性和化学成分。所有分析的肿瘤均由有机和矿物质部分以及水组成。发现磷灰石是矿物质部分的主要成分。研究了各种成分（水、结构羟基、碳酸根离子（CO(3)(2-))和磷酸氢根离子（HPO(4)(2-))）和物理化学参数（磷灰石成熟度和结晶度指数）。在纤维牙骨质瘤中观察到最高的有机/矿物质比，这一发现可以用肿瘤的纤维特征来解释。在牙瘤中发现相对 HPO(4)(2-)含量最低。这种肿瘤的特征是矿物质结晶度指数和结构羟基含量最高。Pindborg 瘤的矿物质部分被发现结晶度差且富含 HPO(4)(2-)。研究中所有样本的相对 CO(3)(2-)含量相似。牙源性肿瘤的光谱研究结果与标准组织化学分析一致。结果表明，ssNMR 的各种技术和 mid-IR 光谱的详细分析相结合，提供了有关良性牙源性钙化肿瘤的有价值信息。
