小鼠B10 B细胞的纯化及免疫表型特征分析
Purification and immunophenotypic characterization of murine B10 B cells.
作者信息
Hong Chao, Gao Xiao-Ming
机构信息
Institute of Biology and Medical Sciences, Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, 215123, Jiangsu Province, China.
出版信息
Methods Mol Biol. 2014;1190:35-44. doi: 10.1007/978-1-4939-1161-5_3.
Regulatory B10 cells represent a rare CD1d(hi)CD5(+) IL-10-secreting B cell subset in mice which is induced to produce IL-10 after 5 h of in vitro stimulation with a combination of B cell mitogen and chemical stimulants. Although B10 cells only constitute 1-2 % of splenic CD19(+) B cells, they play important roles in controlling T cell-mediated immune responses in an antigen-specific and IL-10-dependent manner. The regulatory effects of B10 cells have been demonstrated in multiple mouse models of inflammatory and autoimmune diseases. Herein, we described current methods for identification and purification of CD1d(hi)CD5(+) B10 cells.
调节性B10细胞是小鼠中一种罕见的分泌白细胞介素10（IL-10）的B细胞亚群，其细胞表面CD1d高表达且CD5阳性，在用B细胞有丝分裂原和化学刺激剂组合进行体外刺激5小时后可被诱导产生IL-10。尽管B10细胞仅占脾脏CD19阳性B细胞的1%-2%，但它们以抗原特异性和IL-10依赖性方式在控制T细胞介导的免疫反应中发挥重要作用。B10细胞的调节作用已在多种炎症和自身免疫性疾病的小鼠模型中得到证实。在此，我们描述了目前鉴定和纯化CD1d高表达CD5阳性B10细胞的方法。
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