Zheng Mingke, Xing Chen, Xiao He, Ma Ning, Wang Xiaoqian, Han Gencheng, Chen Guojiang, Hou Chunmei, Shen Beifen, Li Yan, Wang Renxi
Laboratory of Immunology, Institute of Basic Medical Sciences , Beijing , China .
Immunol Invest. 2014;43(7):705-16. doi: 10.3109/08820139.2014.917096. Epub 2014 Jun 20.
Regulatory IL-10-producing CD1d(high)CD5(+)CD19(+) B cells and CD4(+)CD25(+)Foxp3(+) T cells have been found to modulate immune responses in autoimmunity, infection, and cancer, but the interaction between these two cell subsets remains unclear. Through cell culture and flow cytometry (FACS), we analyzed the interaction of regulatory T cells (Tregs) and regulatory B cells (Bregs). A neutralizing antibody was used to determine the role of CD5 and CD72 in maintaining regulatory T and B cell homeostasis. We found that CD19(+)CD5(+)CD1d(hi) Bregs induced expansion of CD4(+)Foxp3(+) Tregs, and CD4(+)CD25(+) Tregs also induced expansion of IL-10-expressing Bregs. Once CD72 or CD5 was blocked, both IL-10-expressing Bregs and CD4(+)Foxp3(+)Tregs were reduced in the different cultures. Finally, FACS analysis demonstrated that Foxp3(+)CD4(+)Treg cells were reduced in CD19(Cre) mice defective of CD5 on the surface of B cells. The study suggests that the interaction of CD5 and CD72 plays a critical role in maintaining regulatory T and B cell homeostasis.
已发现产生调节性白细胞介素-10的CD1d（高表达）CD5（+）CD19（+）B细胞和CD4（+）CD25（+）Foxp3（+）T细胞可调节自身免疫、感染和癌症中的免疫反应，但这两个细胞亚群之间的相互作用仍不清楚。通过细胞培养和流式细胞术（FACS），我们分析了调节性T细胞（Tregs）和调节性B细胞（Bregs）之间的相互作用。使用中和抗体来确定CD5和CD72在维持调节性T细胞和B细胞稳态中的作用。我们发现CD19（+）CD5（+）CD1d（高表达）Bregs可诱导CD4（+）Foxp3（+）Tregs扩增，而CD4（+）CD25（+）Tregs也可诱导表达白细胞介素-10的Bregs扩增。一旦CD72或CD5被阻断，不同培养物中表达白细胞介素-10的Bregs和CD4（+）Foxp3（+）Tregs都会减少。最后，FACS分析表明，B细胞表面缺乏CD5的CD19（Cre）小鼠中Foxp3（+）CD4（+）Treg细胞减少。该研究表明，CD5和CD72的相互作用在维持调节性T细胞和B细胞稳态中起关键作用。
