Rosser Elizabeth C, Mauri Claudia
Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK.
Methods Mol Biol. 2014;1190:183-94. doi: 10.1007/978-1-4939-1161-5_13.
Regulatory B cells (Bregs) have been shown to play a role in the suppression of a wide variety of immune responses. For the study of autoimmune inflammation, experimental models of arthritis have acted as a valuable tool in understanding the function of Bregs in maintaining immune homeostasis. In this chapter, we will focus on the study of transitional-2 marginal zone precursor Bregs (T2-MZPs) in the context of two experimental arthritis models: antigen-induced arthritis (AIA) and collagen-induced arthritis (CIA). We will specifically focus on how to induce arthritis as well as methods for the isolation and functional study of Bregs both in vitro and in vivo.
调节性B细胞（Bregs）已被证明在抑制多种免疫反应中发挥作用。对于自身免疫性炎症的研究，关节炎实验模型已成为理解Bregs在维持免疫稳态中功能的宝贵工具。在本章中，我们将聚焦于在两种实验性关节炎模型——抗原诱导性关节炎（AIA）和胶原诱导性关节炎（CIA）的背景下，对过渡2边缘区前体Bregs（T2-MZPs）的研究。我们将特别关注如何诱导关节炎以及体外和体内Bregs的分离与功能研究方法。
