Ramaswamy Shanmugam, Kuppuswamy Gowthamarajan, Dwarampudi Priyanka, Kadiyala Madhuri, Menta Lokesh, Kannan Elango
Department of Pharmaceutics, JSS College of Pharmacy, (JSS University, Mysore) Udhagamandalam, Tamilnadu, India.
Department of Pharmacology, JSS College of Pharmacy, (JSS University, Mysore) Udhagamandalam, Tamilnadu, India.
Pak J Pharm Sci. 2014 Jul;27(4):901-6.
Curcumin and piperine are proven for their potent medicinal benefits to treat various diseases and they are most commonly used combination in various Indian systems of medicine such as Ayurveda, Siddha and Unani. The objective of the present work is to develop a simultaneous estimation of curcumin and piperine by reverse phase Ultra-fast liquid chromatographic (RP-UFLC) method. The chromatographic separation was performed on a C8 column (250 x 4.6 mm, 5µ i.d.) stationary phase using a mobile phase of 25mM potassium dihydrogen ortho phosphate buffer (pH 3.5) and acetonitrile (30: 70 v/v) at a flow rate of lml/min at detection wave length of 280nm. The calibration curve was plotted in the concentration range of 0-2200ng/ml and found to be linear for both curcumin (r(2)=0.996) and piperine (r(2)=0.999). The method was validated for parameters such as accuracy, sensitivity, precision, linearity, specificity, ruggedness and robustness as per ICH guidelines. The developed simple, precise and specific method can be used as a quality control tool for qualitative and quantitative estimation of curcumin and piperine in various food products, herbal medicines and nutraceuticals.
姜黄素和胡椒碱因其对治疗各种疾病的显著药用功效而得到证实，它们是阿育吠陀、悉达和尤那尼等各种印度医学体系中最常用的组合。本研究的目的是开发一种通过反相超快速液相色谱（RP-UFLC）法同时测定姜黄素和胡椒碱的方法。色谱分离在C8柱（250×4.6mm，内径5µm）固定相上进行，使用25mM磷酸二氢钾缓冲液（pH 3.5）和乙腈（30:70 v/v）的流动相，流速为1ml/min，检测波长为280nm。在0-2200ng/ml的浓度范围内绘制校准曲线，发现姜黄素（r(2)=0.996）和胡椒碱（r(2)=0.999）均呈线性。该方法按照ICH指南对准确性、灵敏度、精密度、线性、特异性、耐用性和稳健性等参数进行了验证。所开发的简单、精确且特异的方法可作为各种食品、草药和营养保健品中姜黄素和胡椒碱定性和定量测定的质量控制工具。
