采用反相液相色谱-多波长检测法分析生物样品中的姜黄素和胡椒碱。

Analysis of curcumin and piperine in biological samples by reversed-phase liquid chromatography with multi-wavelength detection.

作者信息

Rodriguez Elliott L, Zhang Chenhua, Woolfork Ashley G, Li Zhao, Bi Cong, Kaur Harleen, Juritsch Anthony F, Moreau Régis, Hage David S

机构信息

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Jan 1;1162:122487. doi: 10.1016/j.jchromb.2020.122487. Epub 2020 Dec 7.

DOI:10.1016/j.jchromb.2020.122487

PMID:33370686

Abstract

Widely accessible food phytochemicals such as curcumin have been reported to have anti-inflammatory and anticarcinogenic properties. However, curcumin has poor absorption in the gut, and piperine has been of interest as a dietary compound that can enhance curcumin bioavailability. The aim of this study was to develop and optimize a technique using reversed-phase chromatography with multi-wavelength detection for the simultaneous measurement of curcumin and piperine in various biological matrices. Emodin was used as an internal standard. Protein precipitation and liquid-liquid extraction based on acetonitrile provided good recovery of these analytes. A 150 mm × 4.6 mm I.D. Luna C18 column was used under isocratic conditions to separate curcumin, piperine, and emodin with baseline resolution, and with good separation from other sample components, in as little as 4 min. The detection limits for curcumin and piperine were 3 and 7 ng/mL, respectively. This method has been used to quantitate these compounds in samples such as human intestinal epithelial cell lysates and mouse plasma or GI tissues in research aimed at examining the bioavailability of curcumin in the presence of piperine.

摘要

据报道，姜黄素等易于获取的食物中的植物化学物质具有抗炎和抗癌特性。然而，姜黄素在肠道中的吸收较差，胡椒碱作为一种可提高姜黄素生物利用度的膳食化合物受到了关注。本研究的目的是开发并优化一种使用反相色谱和多波长检测的技术，用于同时测定各种生物基质中的姜黄素和胡椒碱。大黄素用作内标。基于乙腈的蛋白质沉淀和液液萃取可使这些分析物获得良好的回收率。在等度条件下，使用一根内径为4.6 mm、长度为150 mm的月旭C18色谱柱，在短短4分钟内就能实现姜黄素、胡椒碱和大黄素的基线分离，与其他样品成分也能实现良好分离。姜黄素和胡椒碱的检测限分别为3 ng/mL和7 ng/mL。在旨在研究姜黄素在胡椒碱存在下生物利用度的研究中，该方法已用于定量人肠上皮细胞裂解物、小鼠血浆或胃肠道组织等样品中的这些化合物。