Ilyas Muhammad, Ahmad Irshad
Biology Department, King Fahd University of Petroleum and Minerals (KFUPM), 34464 Dhahran, Saudi Arabia.
Virol J. 2014 Jul 12;11:127. doi: 10.1186/1743-422X-11-127.
Due to the high rate of asymptomatic infections an advanced screening assay is of prompt importance to be used for the clinical diagnosis of HCV. Early detection of anti HCV is the first step in the management of chronic hepatitis and in the selection of patients needing treatments. In the current study we have first time used the advanced serological diagnostic technique i.e. Chemiluminescent Microparticle Immuno Assay (CMIA) for the detection of HCV infection in Peshawar Pakistan.
A total number of 982 samples were collected among the general public belongs to the different areas of district Peshawar. The samples were centrifuged at high speed to obtain a clear supernatant serum. All the samples were run on Architect system a fully automated immuno analyzer CMIA base technology.
Out of 982 blood samples analyzed in this study, 160 (15.9%) were confirmed to be positive for active HCV infection. The overall prevalence was found to be 13.4%. Gender wise prevalence was recorded to be higher in male (19.1%) than female (12.7%). The age group 21-30 years was identified as the highest risk group among the studied population.
Among the tested samples, overall prevalence of active HCV infection was found to be 13.4% in the general population of Peshawar Pakistan. The young middle aged population of this region was at higher risk of HCV ailments compared to the other age groups.
由于无症状感染率较高，一种先进的筛查检测方法对于丙型肝炎病毒（HCV）的临床诊断至关重要。抗HCV的早期检测是慢性肝炎管理以及选择需要治疗的患者的第一步。在本研究中，我们首次在巴基斯坦白沙瓦使用先进的血清学诊断技术，即化学发光微粒子免疫分析（CMIA）来检测HCV感染。
从白沙瓦地区不同区域的普通人群中总共收集了982份样本。将样本高速离心以获得清澈的上清血清。所有样本均在基于CMIA技术的全自动免疫分析仪Architect系统上进行检测。
在本研究分析的982份血液样本中，有160份（15.9%）被确认为HCV现症感染阳性。总体患病率为13.4%。按性别划分，男性患病率（19.1%）高于女性（12.7%）。在研究人群中，21 - 30岁年龄组被确定为风险最高的组。
在检测的样本中，巴基斯坦白沙瓦普通人群中HCV现症感染的总体患病率为13.4%。与其他年龄组相比，该地区的中青年人群感染HCV疾病的风险更高。
