Eremeeva M E, Lapina E B, Balaeva N M, Ignatovich V F, Belousova L S
Mol Gen Mikrobiol Virusol. 1989 May(5):20-6.
PAAG-electrophoresis of the isogenic pair of Rickettsia prowazekii strains E and Evir lysates demonstrate the similarity in polypeptide tracks. The different electrophoretic mobility of the Mr 30 Kd protein from these strains as compared with the mobility of analogous protein from the standard virulent Breinl strain is registered. In immunoblot experiments the specific rabbit antiserums obtained on the 30th day of infection with the Breinl, E or Evir strains demonstrate the presence of the different main antigens 60 Kd or 70 Kd. The difference evidently reflects the specificity of development of two forms of infection by the strains having different virulence. The surface tris-soluble antigens of Rickettsia prowazekii have the similar polypeptide contents and immunochemical properties. The main component of tris-soluble antigens Mr 130 Kd protein is not strain specific having the common thermolabile epitope.
对普氏立克次体菌株E和Evir裂解物的同基因对进行聚丙烯酰胺凝胶电泳，结果表明多肽条带具有相似性。记录到与标准强毒株布雷茵尔菌株类似蛋白质的迁移率相比，这些菌株中Mr 30 Kd蛋白质的电泳迁移率不同。在免疫印迹实验中，用布雷茵尔、E或Evir菌株感染第30天获得的特异性兔抗血清显示存在不同的主要抗原60 Kd或70 Kd。这种差异显然反映了具有不同毒力的菌株引发的两种感染形式发展的特异性。普氏立克次体的表面三溶性抗原具有相似的多肽含量和免疫化学性质。三溶性抗原的主要成分Mr 130 Kd蛋白质不是菌株特异性的，具有共同的热不稳定表位。
