Emel'ianov V V, Dem'ianova N G, Kalmyrzaev B B, Krasnova M A
Mol Gen Mikrobiol Virusol. 1996 Apr-Jun(2):39-40.
A 29.5 kda outer membrane protein (OmpB) of R. prowazekii virulent Breinl strain is known to differ from its counterpart in attenuated Madrid E strain, while OmpB of this latter one and of its virulent variant EVir coincide in mobility. The infectivity of these strains for macrophages was previously shown to be different as well, and to correlate with their virulence. Previously cloned R. prowazekii Breinl strain, 1.644-bp insert from lambda gtll recombinant expressing as OmpB in inducer-independent fashion was sequenced and used as a query to search for similarity in non-redundant GenBank/EMBL/DDBJ Data Base aided by BLASTX mail server. Extensive homology of inferred 282-aa sequence to peptidyl-propyl cis/trans isomerase C (PPIase C) of E. coli belonging to parvulin family of rotamases (foldases), and related proteins such as Campylobacter jejuni cell binding factor 2 (Cbf2), B. subtilis PrsA, and Lactococcus lactis PrtM has been revealed.
已知普氏立克次体强毒株布雷因尔株的一种29.5千道尔顿外膜蛋白（OmpB）与其减毒株马德里E株的对应蛋白不同，而后者及其强毒株变体EVir的OmpB在迁移率上是一致的。此前已表明，这些菌株对巨噬细胞的感染性也不同，且与其毒力相关。对先前克隆的普氏立克次体布雷因尔株进行了测序，该株来自λgtll重组体的1.644碱基对插入片段，在不依赖诱导剂的情况下表达为OmpB，并以此作为查询序列，借助BLASTX邮件服务器在非冗余GenBank/EMBL/DDBJ数据库中搜索相似性。结果显示，推断的282个氨基酸序列与大肠杆菌的肽基 - 丙基顺/反异构酶C（PPIase C）具有广泛同源性，PPIase C属于旋转异构酶（折叠酶）的小菌素家族，还与空肠弯曲菌细胞结合因子2（Cbf2）、枯草芽孢杆菌PrsA和乳酸乳球菌PrtM等相关蛋白具有同源性。
