Jiao Hong, Zhang Yong, Wang Xiao-Ling
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Mar;30(2):168-71.
To find the characteristic parameters from electrocardiogram (ECG) which is most related to pathological progress, surface ECG was performed in mice at 2, 5, 9 and 13 week post surgery.
Electrocardiogram recordings: The mice were anaesthetized with tribromoethanol (250 mg/kg, i.p.). Lead II surface ECG were acquired by using the Biopac System MP150 at a rate of 5 kHz.
(1) No arrhythmia was observed in Sham and 2-week Band mice. Spontaneous arrhythmias were observed in ECG recordings, with an incidence of 15%, 28% and 63% in 5-, 9- and 13-week Band mice, respectively. Tachyarrhythmias, such as frequent premature ventricular extrasystole, bigeminy, trigeminy, and paroxysmal tachycardia were found. (2) Analysis of ECG recordings revealed a significant prolongation of QT and QTc intervals. Compared with age-matched Sham mice, the increment in QT and QTc intervals was 20.4%, 32.7%, 49.7%, 61.0% and 27.1%, 32.1%, 43.9%, 59.1% at 2, 5, 9 and 13 week, respectively (P < 0.01). (3) The characteristic change in electrocardiogram was on the J wave. The amplitude of J wave was upward in Sham mice, and it was significantly flattened or inverted in Band mice. (4) Except that RR interval at 2 week showed a slight decrease, there was no significant change in PR interval and RR interval in Band mice.
In the present study, we found that the increase incidence of spontaneous arrhythmias, prologation of QT interval and changes in the amplitude of the J wave in the surface ECG during progression of mouse cardiac hypertrophy and failure, and the progressive pattern indicated that a gradual aggravation of the ventricular repolarization delay in this mouse model.
为了找出心电图（ECG）中与病理进展最相关的特征参数，在术后2周、5周、9周和13周对小鼠进行体表心电图检查。
心电图记录：用三溴乙醇（250mg/kg，腹腔注射）麻醉小鼠。使用Biopac系统MP150以5kHz的速率采集II导联体表心电图。
（1）假手术组和术后2周束带组小鼠未观察到心律失常。在心电图记录中观察到自发性心律失常，术后5周、9周和13周束带组小鼠的发生率分别为15%、28%和63%。发现了快速性心律失常，如频发室性早搏、二联律、三联律和阵发性心动过速。（2）心电图记录分析显示QT和QTc间期显著延长。与年龄匹配的假手术组小鼠相比，术后2周、5周、9周和13周时QT和QTc间期的增加分别为20.4%、32.7%、49.7%、61.0%和27.1%、32.1%、43.9%、59.1%（P<0.01）。（3）心电图的特征性变化在于J波。假手术组小鼠J波振幅向上，而束带组小鼠J波明显变平或倒置。（4）除术后2周RR间期略有下降外，束带组小鼠PR间期和RR间期无显著变化。
在本研究中，我们发现在小鼠心脏肥大和衰竭进展过程中，体表心电图自发性心律失常发生率增加、QT间期延长以及J波振幅变化，且这种进展模式表明该小鼠模型中心室复极延迟逐渐加重。
