Lou Hongfei, Fu Yujing, Wang Chengshuo, Wang Yang, Zhang Luo
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Nasal Diseases, Beijing Institute of Otorhinolaryngology, Beijing 100730, China.
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Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 May;49(5):390-4.
To investigate the influence of maternal atopy on cord blood effector T cells and to identify these biologic markers as predictors of atopic dermatitis (AD).
Seventy mother-infant pairs were recruited in this prospective birth cohort study. Suspected factors for allergy, including maternal allergic history, total serum IgE, and maternal age at birth, were collected. Mother peripheral blood samples and cord blood were obtained and assayed for the percentage of interferon-γ (IFN-γ) and interleukin 4 (IL-4) producing T cells(Th1 and Th2 respectively) using flow cytometry. Their offspring at the age of 2 years old were evaluated by their dermatologist whether they had AD. Statistical analysis was performed using multiple logistic regression models and receiver-operating characteristic curve was employed to predict atopic dermatitis.
Twenty-one allergic and 49 nonallergic mothers were recruited in this study. During the first two years of life, 15.7% children (n = 11) developed a physician-diagnosed AD (all children were the only child in the family). In group with maternal allergic rhinitis, a significantly increased percentage of Th2 was observed in peripheral blood of mother (7.10[1.18;16.1]% vs. 0.37[0.25;0.72]%, U = 10.0, P < 0.05) and cord blood of newborns (1.02[0.57;1.34]% vs. 0.21[0.15;0.42]%, U = 127.5, P < 0.05), respectively. Maternal atopic history did not affect the percentage of Th1 cells in cord blood (0.69[0.40;1.12]% vs.0.50[0.31;0.66]%, U = 361.0, P > 0.05). Children with reduced Th1/Th2 ratio in cord blood had a higher risk to develop AD (OR = 1.72, P = 0.001) . The model including Th1/Th2, maternal allergy, maternal age at birth and maternal total IgE showed high ability to discriminate children with and without AD. AUC was 0.907 (95% CI: 0.804-1.011, P < 0.001).
Elevated IL-4⁺CD4⁺ T cells in cord blood were of relevance with maternal allergic history. Imbalance between Th1 cell and Th2 cell at birth are associated with maternal allergy and promoted subsequent AD development.
探讨母亲特应性对脐血效应T细胞的影响，并确定这些生物学标志物作为特应性皮炎（AD）的预测指标。
在这项前瞻性出生队列研究中招募了70对母婴。收集疑似过敏因素，包括母亲过敏史、血清总IgE和母亲分娩时年龄。采集母亲外周血样本和脐血，采用流式细胞术检测产生干扰素-γ（IFN-γ）和白细胞介素4（IL-4）的T细胞（分别为Th1和Th2）百分比。其2岁的后代由皮肤科医生评估是否患有AD。使用多元逻辑回归模型进行统计分析，并采用受试者工作特征曲线预测特应性皮炎。
本研究招募了21名过敏母亲和49名非过敏母亲。在生命的前两年中，15.7%的儿童（n = 11）被医生诊断为AD（所有儿童均为独生子女）。在患有母亲过敏性鼻炎的组中，母亲外周血（7.10[1.18；16.1]%对0.37[0.25；0.72]%，U = 10.0，P < 0.05）和新生儿脐血（1.02[0.57；1.34]%对0.21[0.15；0.42]%，U = 127.5，P < 0.05）中Th2百分比显著增加。母亲特应性病史不影响脐血中Th1细胞百分比（0.69[0.40；1.12]%对0.50[0.31；0.66]%，U = 361.0，P > 0.05）。脐血中Th1/Th2比值降低 的儿童患AD的风险更高（OR = 1.72，P = 0.001）。包括Th1/Th2、母亲过敏、母亲分娩时年龄和母亲总IgE的模型显示出区分患AD和未患AD儿童的高能力。曲线下面积为0.907（95%CI：0.804 - 1.011，P < 0.001）。
脐血中IL-4⁺CD4⁺ T细胞升高与母亲过敏史有关。出生时Th1细胞和Th2细胞之间的失衡与母亲过敏有关，并促进随后AD的发展。
