Cheng Chih-Chien, Chung Chih-Ang, Su Li-Chiu, Chien Chih-Cheng, Cheng Yu-Che
Department of Mechanical Engineering, National Central University, Jhongli, Taiwan; Department of Obstetrics and Gynecology, Sijhih Cathay General Hospital, Taipei, Taiwan; Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan.
Department of Mechanical Engineering, National Central University, Jhongli, Taiwan; Institute of Biomedical Engineering, National Central University, Jhongli, Taiwan.
Taiwan J Obstet Gynecol. 2014 Jun;53(2):187-92. doi: 10.1016/j.tjog.2014.04.011.
Stem cells offer great potential for clinical therapeutic use because of their ability to rejuvenate and to differentiate into numerous types of cells. We isolated multipotent cells from the human term placenta that were capable of differentiation into cells of all three germ layers.
We examined the ability of these placenta-derived multipotent cells (PDMCs) to differentiate into osteoblasts (OBs) or OB-like cells. The PDMCs were treated with osteogenic medium (OM) consisting of dexamethasone, β-glycerol phosphate, and ascorbic acid. At sequential time intervals (0 day, 3 days, 6 days, 9 days, and 12 days) we measured several parameters. These included alkaline phosphatase (ALP) activity, alizarin red staining (ARS) to measure calcium deposition, messenger RNA (mRNA) expressions of osteogenesis-related transcription factor (Cbfa1), and calcium coordination protein (osteocalcin). These variables were used as indicators of PDMC osteodifferentiation.
We showed that ALP activity in the early stage of differentiation and calcium deposition were both significantly increased in PDMCs after OM induction. Moreover, the Cbfa1 and osteocalcin gene expressions were upregulated. The results suggested that OM induced an osteodifferentiation potential in PDMCs.
PDMC-derived osteocytes provide a useful model to evaluate the mechanisms of key biomolecules and bioengineering processes.
干细胞因其具有恢复活力及分化为多种类型细胞的能力，在临床治疗应用中具有巨大潜力。我们从足月人胎盘中分离出了能够分化为所有三个胚层细胞的多能细胞。
我们检测了这些胎盘来源的多能细胞（PDMCs）分化为成骨细胞（OBs）或类成骨细胞的能力。将PDMCs用由地塞米松、β-甘油磷酸和抗坏血酸组成的成骨培养基（OM）处理。在连续的时间间隔（0天、3天、6天、9天和12天）测量了几个参数。这些参数包括碱性磷酸酶（ALP）活性、用于测量钙沉积的茜素红染色（ARS）、成骨相关转录因子（Cbfa1）的信使核糖核酸（mRNA）表达以及钙结合蛋白（骨钙素）。这些变量被用作PDMCs骨分化的指标。
我们发现，经OM诱导后，PDMCs分化早期的ALP活性和钙沉积均显著增加。此外，Cbfa1和骨钙素基因表达上调。结果表明，OM诱导了PDMCs的骨分化潜能。
PDMC来源的骨细胞为评估关键生物分子和生物工程过程的机制提供了一个有用的模型。
