Department of Mechanical Engineering, National Central University, Jhongli, Taiwan; Department of Obstetrics/Gynecology, Taipei City Hospital, Taipei, Taiwan; School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan.
Department of Mechanical Engineering, National Central University, Jhongli, Taiwan.
Taiwan J Obstet Gynecol. 2022 Mar;61(2):270-276. doi: 10.1016/j.tjog.2022.02.014.
We tested the osteoblastic differentiation effects caused by physical stimulation such as hydrostatic pressure using placenta-derived multipotent cells.
The placenta-derived multipotent cells (PDMCs) were treated with osteogenic medium to induce PDMCs differentiation into osteoblast-like cells. The induced PDMCs were stimulated using hydrostatic pressure at a magnitude of 30 kPa for 1 h/day for up to 12 days. The calcium deposition monitored by Alizarin Red staining and the calcium content of each experimental group were quantified.
The results demonstrated both the calcium deposition and concentration were elevated through hydrostatic pressure stimulation. Moreover, in order to indicate of PDMC osteodifferentiation, RT-qPCR analysis were performed and mRNA expression of osteoblast differentiation markers (type I collagen, alkaline phosphatase, RUNX2, and BGLAP), the bone morphogenetic protein family (BMP1-7) and BMP receptors (BMPR1A, BMPR1B, and BMPR2) were examined. Among them, the mRNA levels of RUNX2, COL1A1, BMP1, BMP3, and BMPR1A increased significantly in the hydrostatic-pressure-stimulated groups, whereas BGLAP, ALP, BMP2, BMP6, BMPR1B, and BMPR2 exhibited a slight upregulation between the control and experimental groups, indicating the specific signal route induced by hydrostatic pressure on PDMCs.
Our results revealed the beneficial effects of stem cells stimulated using hydrostatic pressure, which could enhance calcium deposition considerably and facilitate osteodifferentiation, and the results may be applied to tissue regeneration in the near future.
我们通过静水压力等物理刺激测试了胎盘来源多能细胞的成骨分化作用。
将胎盘来源多能细胞（PDMCs）用成骨培养基处理，诱导 PDMCs 分化为成骨样细胞。用 30 kPa 的静水压力刺激诱导的 PDMCs，每天 1 小时，持续 12 天。通过茜素红染色监测钙沉积，并对每个实验组的钙含量进行定量。
结果表明，静水压力刺激可提高钙沉积和浓度。此外，为了指示 PDMC 成骨分化，进行了 RT-qPCR 分析，检测成骨分化标志物（I 型胶原、碱性磷酸酶、RUNX2 和 BGLAP）、骨形态发生蛋白家族（BMP1-7）和 BMP 受体（BMPR1A、BMPR1B 和 BMPR2）的 mRNA 表达。其中，RUNX2、COL1A1、BMP1、BMP3 和 BMPR1A 的 mRNA 水平在静水压力刺激组中显著增加，而 BGLAP、ALP、BMP2、BMP6、BMPR1B 和 BMPR2 在对照组和实验组之间略有上调，表明静水压力对 PDMCs 诱导的特定信号途径。
我们的结果揭示了干细胞受到静水压力刺激的有益作用，可显著增加钙沉积并促进成骨分化，这些结果可能在不久的将来应用于组织再生。
