Ruta Lavinia L, Popa Valentina C, Nicolau Ioana, Danet Andrei F, Iordache Virgil, Neagoe Aurora D, Farcasanu Ileana C
University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania.
University of Bucharest, Faculty of Biology, Spl. Independentei 91-95, 050095 Bucharest, Romania.
FEBS Lett. 2014 Aug 25;588(17):3202-12. doi: 10.1016/j.febslet.2014.07.001. Epub 2014 Jul 10.
The involvement of Ca(2+) in the response to high Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), and Hg(2+) was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca(2+) when exposed to Cd(2+), and to a lesser extent to Cu(2+), but not to Mn(2+), Co(2+), Ni(2+), Zn(2+), or Hg(2+). The response to high Cd(2+) depended mainly on external Ca(2+) (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca(2+) (released into the cytosol through the Yvc1p channel). The adaptation to high Cd(2+) was influenced by perturbations in Ca(2+) homeostasis. Thus, the tolerance to Cd(2+) often correlated with sharp Cd(2+)-induced cytosolic Ca(2+) pulses, while the Cd(2+) sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca(2+).
在酿酒酵母中研究了Ca(2+)在对高浓度Mn(2+)、Co(2+)、Ni(2+)、Cu(2+)、Zn(2+)、Cd(2+)和Hg(2+)的反应中的作用。酵母细胞在暴露于Cd(2+)时，通过胞质Ca(2+)的急剧增加做出反应，对Cu(2+)的反应程度较小，但对Mn(2+)、Co(2+)、Ni(2+)、Zn(2+)或Hg(2+)没有反应。对高浓度Cd(2+)的反应主要依赖于外部Ca(2+)（通过Cch1p/Mid1p通道转运），但也依赖于液泡Ca(2+)（通过Yvc1p通道释放到细胞质中）。对高浓度Cd(2+)的适应性受到Ca(2+)稳态扰动的影响。因此，对Cd(2+)的耐受性通常与Cd(2+)诱导的胞质Ca(2+)脉冲急剧相关，而Cd(2+)敏感性则伴随着无法迅速恢复低水平的胞质Ca(2+)。
