Gogianu Larisa Ioana, Ruta Lavinia Liliana, Farcasanu Ileana Cornelia
Doctoral School of Biology, Faculty of Biology, University of Bucharest, Splaiul Independenței 91-95, 050095 Bucharest, Romania.
National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Str. 126A, 077190 Voluntari, Romania.
Molecules. 2024 Nov 28;29(23):5627. doi: 10.3390/molecules29235627.
Recombinant aequorin has been extensively used in mammalian and plant systems as a powerful tool for calcium monitoring. While aequorin has also been widely applied in yeast research, a notable gap exists in the literature regarding comprehensive reviews of these applications. This review aims to address that gap by providing an overview of how aequorin has been used to explore calcium homeostasis, signaling pathways, and responses to stressors, heavy metals, and toxic compounds in . We also discuss strategies for further developing the aequorin system in yeast, with particular emphasis on its use as a model for human calcium signaling studies, such as the reproduction of the mitochondrial calcium uniporter. By highlighting previous research and pinpointing potential future applications, we discuss the untapped potential of aequorin in yeast for drug screening, environmental toxicity testing, and disease-related studies.
重组水母发光蛋白已作为一种用于钙监测的强大工具在哺乳动物和植物系统中广泛使用。虽然水母发光蛋白也已广泛应用于酵母研究，但文献中关于这些应用的全面综述存在显著空白。本综述旨在通过概述水母发光蛋白如何用于探索酵母中的钙稳态、信号通路以及对应激源、重金属和有毒化合物的反应来填补这一空白。我们还讨论了进一步开发酵母中水母发光蛋白系统的策略，特别强调其作为人类钙信号研究模型的用途，例如线粒体钙单向转运体的重现。通过突出先前的研究并指出潜在的未来应用，我们讨论了水母发光蛋白在酵母中用于药物筛选、环境毒性测试和疾病相关研究的未开发潜力。
