Wang H M, Jen C J
Department of Physiology, College of Medicine, National Cheng-Kung University, Tainan, Taiwan.
Thromb Res. 1989 May 1;54(3):165-75. doi: 10.1016/0049-3848(89)90224-7.
The dynamic interactions between platelets and fibrinogen-coated surfaces were investigated by using a tube-flow device and a rotating rod device. The net platelet accumulation to the fibrinogen-coated surfaces in both devices reached maximal values of 30-40 platelets/1000 um2. This adhesion phenomenon was completely inhibited by EDTA. In the tube-flow device, the density of platelets adhered to the tube surface increased with flow time and decreased with distance from the tube inlet. These adhered platelets were difficult to be washed off the tube surface and showed little turnover. The platelet accumulation kinetics in a rotating rod device increased with rotation speed from 300 rpm to 1200 rpm. At 1200 rpm, about half of these platelets adhered to the rod were exchangeable with platelets in the suspension whereas the rest of them were permanent. The detachment of platelets from the rod surface depended on the presence of erythrocytes and platelets in the suspension and it was facilitated by the presence of EDTA in the suspension. These observations suggest that the turnover of platelets adhered to fibrinogen-coated surfaces depends on experimental conditions.
通过使用管流装置和旋转杆装置研究了血小板与纤维蛋白原包被表面之间的动态相互作用。在这两种装置中，血小板在纤维蛋白原包被表面的净积累量达到了30 - 40个血小板/1000平方微米的最大值。这种粘附现象被EDTA完全抑制。在管流装置中，粘附在管壁表面的血小板密度随流动时间增加，随距管入口距离减小。这些粘附的血小板很难从管壁表面冲洗掉，且周转很少。旋转杆装置中的血小板积累动力学随转速从300转/分钟增加到1200转/分钟而增加。在1200转/分钟时，粘附在杆上的这些血小板中约一半可与悬浮液中的血小板交换，而其余的则是永久性的。血小板从杆表面的脱离取决于悬浮液中红细胞和血小板的存在，并且悬浮液中EDTA的存在促进了这种脱离。这些观察结果表明，粘附在纤维蛋白原包被表面的血小板的周转取决于实验条件。
