Feuerstein I A, Kush J
J Biomech Eng. 1986 Feb;108(1):49-53. doi: 10.1115/1.3138579.
The interaction of fluorescently labeled blood platelets with fibrinogen-coated glass was studied in Poiseuille flow at 3 wall shear rates, 40, 80 and 944 s-1. Observations were made via video-microscopy at a distance of 0.5 cm from a tube's entrance over a 1370 microns 2 portion of luminal area. The rates of arrival and detachment, and the net rate of adhesion of cells increased nonlinearly with flow rate. The fraction of arriving cells, first contacts, which adhered without subsequent movement and the fraction of arriving cells which adhered, moved to new positions and then remained adherent, were maximal at 80 s-1. For platelets which adhere and then move to a number of new positions, the likelihood of permanent adhesion is greater than 85 percent. The adhesion process is one in which 40-60 percent of cells permanently adhere on first contact with an additional 30 percent adhering after several moves along the surface. Cells contacting where a platelet was previously adherent had a greater chance of adhering than they would on an unaltered fibrinogen surface. The efficiency of platelet adhesion is greater for second contacts than for first contacts on unaltered fibrinogen coated surface.
在泊肃叶流中，研究了荧光标记的血小板与纤维蛋白原包被玻璃在3种壁面剪切速率（40、80和944 s⁻¹）下的相互作用。通过视频显微镜在距管入口0.5 cm处、管腔面积1370平方微米的区域内进行观察。细胞的到达和脱离速率以及净黏附速率随流速呈非线性增加。在80 s⁻¹时，到达的细胞中，无后续移动而黏附的首次接触细胞比例以及黏附后移动到新位置并保持黏附的到达细胞比例均达到最大值。对于那些黏附后移动到多个新位置的血小板，永久黏附的可能性大于85%。黏附过程中，40% - 60%的细胞在首次接触时永久黏附，另有30%的细胞在沿表面多次移动后黏附。与先前有血小板黏附的部位接触的细胞，比在未改变的纤维蛋白原表面有更大的黏附机会。在未改变的纤维蛋白原包被表面，血小板第二次接触时的黏附效率高于第一次接触。
