Bhavsar Rital B, Tsonis Panagiotis A
Department of Biology and Center for Tissue Regeneration and Engineering, University of Dayton, Dayton, Ohio, United States of America.
PLoS One. 2014 Jul 14;9(7):e102510. doi: 10.1371/journal.pone.0102510. eCollection 2014.
From the cocktail of four factors that were able to induce pluripotent stem cells from differentiated cells, Oct-4, c-Myc, Sox-2 and Klf4, only Oct-4 was not expressed during regeneration in newts. To explore the possible action of this stemness factor we developed an assay where we introduced exogenous Oct-4 protein to an in vitro system for lens regeneration in newts. We found that exogenous Oct-4 inhibits differentiation of iris pigmented epithelial cells into lens cells and also regulates Sox-2 and Pax-6, both important players during lens development. Thus, presence of Oct-4 hinders transdifferentiation of iris cells.
在能够将分化细胞诱导为多能干细胞的四种因子（Oct-4、c-Myc、Sox-2和Klf4）的组合中，只有Oct-4在蝾螈再生过程中不表达。为了探究这种干性因子的可能作用，我们开发了一种检测方法，即将外源性Oct-4蛋白引入蝾螈晶状体再生的体外系统中。我们发现外源性Oct-4抑制虹膜色素上皮细胞向晶状体细胞的分化，并且还调节Sox-2和Pax-6，这两者都是晶状体发育过程中的重要参与者。因此，Oct-4的存在会阻碍虹膜细胞的转分化。
