Ramírez-Expósito María Jesús, Sánchez-López Estefanía, Cueto-Ureña Cristina, Dueñas Basilio, Carrera-González Pilar, Navarro-Cecilia Joaquín, Mayas María Dolores, Arias de Saavedra José M, Sánchez-Agesta Rafael, Martínez-Martos José M
Experimental and Clinical Physiopathology Research Group BIO296, Department of Health Sciences, School of Experimental and Health Sciences, University of Jaen, Campus Las Lagunillas, E-23071 Jaen, Spain.
Experimental and Clinical Physiopathology Research Group BIO296, Department of Health Sciences, School of Experimental and Health Sciences, University of Jaen, Campus Las Lagunillas, E-23071 Jaen, Spain.
Exp Gerontol. 2014 Oct;58:34-42. doi: 10.1016/j.exger.2014.07.006. Epub 2014 Jul 11.
We evaluate here the redox status in pre- and post-menopausal healthy women and in women with breast cancer in order to understand the consequences of the hormonal alterations of menopause for the oxidative stress status, its modifications with breast cancer and the influence of neoadjuvant chemotherapy (NC). To that, serum oxidative stress parameters (total antioxidant capacity, lipid peroxidation and protein oxidation), non-enzyme antioxidant defenses (total glutathione, uric acid and bilirubin) and enzyme antioxidant defenses (superoxide dismutase, catalase and glutathione peroxidase activities) were measured in healthy women and in women with breast cancer divided according to their menopausal status and that received or not NC. Circulating estradiol, progesterone, FSH and LH were also analyzed. We found that menopause itself modifies the redox status of healthy women, being most of these differences also reflected in women with breast cancer. However, several changes occur as a consequence of the disease. Furthermore, NC increases oxidative damage, decreases antioxidant defenses and eliminates the differences found in menopause. We conclude that the normal redox balance is disrupted by breast cancer but is also affected by the hormonal status promoted by menopause. In fact, NC nullifies the differences found between pre- and postmenopausal women in several antioxidant defense systems.
我们在此评估绝经前和绝经后健康女性以及乳腺癌女性的氧化还原状态，以了解绝经激素变化对氧化应激状态的影响、乳腺癌对其的改变以及新辅助化疗（NC）的影响。为此，我们测量了健康女性和乳腺癌女性血清中的氧化应激参数（总抗氧化能力、脂质过氧化和蛋白质氧化）、非酶抗氧化防御（总谷胱甘肽、尿酸和胆红素）以及酶抗氧化防御（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性），这些女性根据绝经状态进行分组，并接受或未接受NC治疗。同时还分析了循环中的雌二醇、孕酮、促卵泡激素（FSH）和促黄体生成素（LH）。我们发现，绝经本身会改变健康女性的氧化还原状态，其中大部分差异也反映在乳腺癌女性中。然而，疾病也会导致一些变化。此外，NC会增加氧化损伤，降低抗氧化防御能力，并消除绝经时发现的差异。我们得出结论，正常的氧化还原平衡会被乳腺癌破坏，但也会受到绝经所带来的激素状态的影响。事实上，NC消除了绝经前和绝经后女性在几种抗氧化防御系统中发现的差异。
