a Experimental and Clinical Physiopathology Research Group CTS-1039, Department of Health Sciences, School of Experimental and Health Sciences , University of Jaén , Jaén , Spain.
b Unit of Breast Pathology , Complejo Hospitalario de Jaén , Jaén , Spain.
Ups J Med Sci. 2017 Nov;122(4):207-216. doi: 10.1080/03009734.2017.1403522. Epub 2017 Dec 21.
Lymphatic metastasis is regulated in multiple steps including the transit of tumor cells via the lymphatic vessels and the successful seeding in draining lymph nodes. Thus, several molecular signals and cellular changes must be involved in this complex process to facilitate tumor cell entry, colonization, and survival in the lymph node. To our knowledge, the present work explores, for the first time in the literature, the redox status (oxidative stress parameters and enzymatic and non-enzymatic antioxidant defense systems) in the sentinel lymph node (SLN) of women with breast cancer.
SLNs from 75 women with breast cancer were identified using the one-step nucleic acid amplification (OSNA) method as negative (n = 43), with micrometastases (n = 13), or with macrometastases (n = 19). It will allow us to gain knowledge about the pro-oxidant/antioxidant mechanisms involved in the processes of distant metastases in breast cancer and also to assess whether these parameters may be alternative techniques for staging.
We found different levels of lipid peroxidation in SLNs with micrometastases (increased) and macrometastases (decreased), a decrease in carbonyl group content in SLNs with macrometastases only, and an increase in total antioxidant capacity (TAC) in SNLs with micrometastases and macrometastases. A decrease in the levels of reduced glutathione (GSH) also appears in the SLNs with macrometastases only. Finally, we show increased levels of superoxide dismutase (SOD) and catalase (CAT) activity in SLNs with micrometastases and macrometastases, and decreased levels of glutathione peroxidase (GPx) activity in SNLs with macrometastases but not with micrometastases.
Redox status of lymph node microenvironment participates in the progression of metastatic breast cancer.
淋巴转移是一个多步骤的过程，包括肿瘤细胞通过淋巴管的转移以及在引流淋巴结中的成功定植。因此，在这个复杂的过程中，必须有几个分子信号和细胞变化来促进肿瘤细胞进入、定植和在淋巴结中存活。据我们所知，目前的工作首次在文献中探讨了乳腺癌患者前哨淋巴结（SLN）的氧化还原状态（氧化应激参数以及酶和非酶抗氧化防御系统）。
使用一步核酸扩增（OSNA）方法鉴定了 75 名乳腺癌女性的 SLN，结果为阴性（n=43）、有微转移（n=13）或有宏转移（n=19）。这将使我们能够了解参与乳腺癌远处转移过程的促氧化剂/抗氧化剂机制，并评估这些参数是否可能是分期的替代技术。
我们发现有微转移的 SLN 中脂质过氧化水平不同（增加），有宏转移的 SLN 中脂质过氧化水平降低（减少），只有有宏转移的 SLN 中羰基含量降低，有微转移和宏转移的 SLN 中总抗氧化能力（TAC）增加。只有有宏转移的 SLN 中还原型谷胱甘肽（GSH）水平也降低。最后，我们发现有微转移和宏转移的 SLN 中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性增加，有宏转移的 SLN 中谷胱甘肽过氧化物酶（GPx）活性降低，但有微转移的 SLN 中无此变化。
淋巴结微环境的氧化还原状态参与了转移性乳腺癌的进展。
