[合成人血管生成素基因在大肠杆菌中表达裂解型β-半乳糖苷酶-血管生成素蛋白]
[Expression of synthetic human angiogenin gene in E. coli in cleaved beta-galactosidase-angiogenin protein].
作者信息
Kovalenko S P, Lisnyak I A, Mertvetsov N P
出版信息
Bioorg Khim. 1989 Apr;15(4):492-8.
PMID:2502122
Abstract
A synthetic gene coding for human angiogenin was cloned in pUR290 plasmid in frame with beta-galactosidase, both parts of the resultant fused protein being joined through an Asp-Pro sequence. The fused protein, synthesised in E. coli cells upon IPTG induction and isolated as inclusion bodies, possessed angiogenic activity on the chick chorioallantois membrane and was cleaved upon acid treatment to yield free angiogenin.
摘要
编码人血管生成素的合成基因被克隆到pUR290质粒中，与β-半乳糖苷酶读框一致，所得融合蛋白的两部分通过天冬氨酸-脯氨酸序列连接。融合蛋白在IPTG诱导下于大肠杆菌细胞中合成，并作为包涵体分离出来，在鸡胚绒毛尿囊膜上具有血管生成活性，经酸处理后被切割产生游离的血管生成素。
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