Chakhmakhcheva O G, Mirskikh O V, Chiong N H, Efimov V A
Bioorg Khim. 1987 Mar;13(3):350-8.
With the use of synthetic DNA fragments, a set of new plasmid vectors has been obtained. The vectors provided high level expression of peptides and small proteins in E. coli as fusions with fragments of beta-galactosidase of various length. These vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. The yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total amount of cellular protein.
通过使用合成DNA片段，获得了一组新的质粒载体。这些载体能使肽和小蛋白质在大肠杆菌中与不同长度的β-半乳糖苷酶片段融合而实现高水平表达。这些载体被用于实现细菌视紫红质功能活性片段的合成基因的表达。由β-半乳糖苷酶和细菌视紫红质片段组成的杂合蛋白产量占细胞总蛋白量的5%-30%。
