[人血管生成素合成基因在大肠杆菌细胞中的表达]
[Expression of synthetic gene for human angiogenin in E. coli cells].
作者信息
Nikonova A A, Seregin S V, Chikaev N A, Mishin V P, Babkina I N, Mertvetsov N P
出版信息
Bioorg Khim. 1996 Dec;22(12):891-3.
PMID:9054338
Abstract
A synthetic gene for human angiogenin was cloned into pRTang under control of the Proteus mirabilis recA promoter. After induction with mitomycin C, Escherichia coli cells transformed with pRTang produced an additional 14.2-kDa protein. According to electrophoretic and immunochemical analyses, this protein was identical to human angiogenin.
摘要
将人血管生成素的合成基因克隆到奇异变形杆菌recA启动子控制下的pRTang载体中。用丝裂霉素C诱导后，用pRTang转化的大肠杆菌细胞产生了一种额外的14.2 kDa蛋白。根据电泳和免疫化学分析，该蛋白与人血管生成素相同。
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