Liu Yinglan, Jiang Xiaoxin, Zeng Yong, Zhou Hui, Yang Jing, Cao Renxian
Department of Endocrinology, The First Affiliated Hospital of Nanhua University, No. 69 Chuanshan Road, Hengyang, 421001, Hunan Province, China.
Histochem Cell Biol. 2014 Dec;142(6):685-91. doi: 10.1007/s00418-014-1248-4. Epub 2014 Jul 16.
High levels of aldehyde dehydrogenase (ALDH) activity have been regarded as a specific feature of progenitor cells and stem cells. Hence, as an indicator of ALDH activity, aldefluor fluorescence has been widely used for the identification and isolation of stem and progenitor cells. ALDH activity was recently detected in embryonic mouse pancreas, and specifically and exclusively in adult centroacinar and terminal duct cells, suggesting that these duct cells may harbor cells of endocrine and exocrine differentiation potential in the adult pancreas. Here, we report the presence of aldefluor+ beta-cells in a beta-cell proliferation model, partial pancreatectomy. The aldefluor+ beta-cells are essentially all positive for Ki-67 and expressed high levels of cell-cycle activators such as CyclinD1, CyclinD2, and CDK4, suggesting that they are mitotic cells. Our data thus reveal a potential change in ALDH activity of proliferating beta-cells, which provides a novel method for the isolation and analysis of proliferating beta-cells. Moreover, our data also suggest that aldefluor lineage-tracing is not a proper method for analyzing progenitor or stem activity in the adult pancreas.
高水平的醛脱氢酶（ALDH）活性被视为祖细胞和干细胞的一个特定特征。因此，作为ALDH活性的一个指标，醛荧光已被广泛用于干细胞和祖细胞的鉴定与分离。最近在胚胎期小鼠胰腺中检测到了ALDH活性，且在成年胰腺的中央腺泡细胞和终末导管细胞中特异性且唯一地检测到，这表明这些导管细胞可能含有成年胰腺中具有内分泌和外分泌分化潜能的细胞。在此，我们报告在β细胞增殖模型——部分胰腺切除术中存在醛荧光阳性的β细胞。醛荧光阳性的β细胞基本上都对Ki-67呈阳性，并表达高水平的细胞周期激活因子，如细胞周期蛋白D1、细胞周期蛋白D2和细胞周期蛋白依赖性激酶4，这表明它们是有丝分裂细胞。因此，我们的数据揭示了增殖β细胞ALDH活性的潜在变化，这为增殖β细胞的分离和分析提供了一种新方法。此外，我们的数据还表明，醛荧光谱系追踪不是分析成年胰腺中祖细胞或干细胞活性的合适方法。
