Rios-Yuil Jose M, Herrera-Gonzalez Norma E, Aguilar-Faisal Jose L, Lara-Padilla Eleazar, Mercadillo-Perez Patricia, Moreno-Lopez Luis M, Marquez-Ramirez Alejandro K, Saldana-Patino Azael, Rubio-Gayosso Ivan
Departments of Dermatology and Pathology of the Panamanian Social Security (Caja de Seguro Social) and San Fernando Hospital (Clinica Hospital San Fernando), Panama City, Panama; Postgraduate studies and Research Section, Superior School of Medicine, Instituto Politecnico Nacional, Mexico City, Mexico, .
Indian J Dermatol Venereol Leprol. 2014 Jul-Aug;80(4):381. doi: 10.4103/0378-6323.136992.
Information is scarce about the presence of molecular alterations related to human papillomavirus (HPV) infection in squamous cell carcinomas of the genital skin and about the effect of this infection in the number of Langerhans cells present in these tumors.
To determine the presence of HPV in genital skin squamous cell carcinomas and to see the relationship between HPV infection and changes in the expression of Ki-67 antigen (Ki-67), p53 protein (p53), retinoblastoma protein (pRb) and E-cadherin and to alterations in Langerhans cell density, if any.
A descriptive, comparative, retrospective and cross-sectional study was performed with all the cases diagnosed as squamous cell carcinomas of the genital skin at the Dermatopathology Service from 2001 to 2011. The diagnosis was verified by histopathological examination. The presence of HPV was examined using chromogenic in situ hybridization, and protein expression was studied via immunohistochemical analysis.
The 34 cases studied were verified as squamous cell carcinomas and 44.1% were HPV positive. The degree of expression of pRb was 17.50% ±14.11% (mean ± SD) in HPV-positive cases and 29.74% ±20.38% in HPV-negative cases (P = 0.0236). The degree of expression of Ki-67 was 47.67% ±30.64% in HPV-positive cases and 29.87% ±15.95% in HPV-negative cases (P = 0.0273).
HPV infection was related to lower pRb expression and higher Ki-67 expression in comparison with HPV negative samples. We could not find a relationship between HPV infection and the degree of expression of p53 and E-cadherin or with Langerhans cell density.
关于生殖器皮肤鳞状细胞癌中与人类乳头瘤病毒（HPV）感染相关的分子改变情况，以及这种感染对这些肿瘤中朗格汉斯细胞数量的影响，相关信息十分匮乏。
确定HPV在生殖器皮肤鳞状细胞癌中的存在情况，并观察HPV感染与Ki-67抗原（Ki-67）、p53蛋白（p53）、视网膜母细胞瘤蛋白（pRb）和E-钙黏蛋白表达变化之间的关系，以及与朗格汉斯细胞密度改变（若有）之间的关系。
对2001年至2011年在皮肤病理学服务部诊断为生殖器皮肤鳞状细胞癌的所有病例进行了一项描述性、对比性、回顾性和横断面研究。通过组织病理学检查验证诊断。使用显色原位杂交检测HPV的存在，并通过免疫组织化学分析研究蛋白表达。
研究的34例病例被确认为鳞状细胞癌，44.1%为HPV阳性。HPV阳性病例中pRb的表达程度为17.50%±14.11%（均值±标准差），HPV阴性病例中为29.74%±20.38%（P = 0.0236）。HPV阳性病例中Ki-67的表达程度为47.67%±30.64%，HPV阴性病例中为29.87%±15.95%（P = 0.0273）。
与HPV阴性样本相比，HPV感染与较低的pRb表达和较高的Ki-67表达有关。我们未发现HPV感染与p53和E-钙黏蛋白的表达程度或与朗格汉斯细胞密度之间存在关联。
