Moradpour Nona, Borji Hassan, Razmi Gholamreza, Kazemi Hossein, Maleki Mohsen
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, 9177948974, Mashhad, Islamic Republic of Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran.
J Parasit Dis. 2014 Sep;38(3):289-92. doi: 10.1007/s12639-013-0243-3. Epub 2013 Jan 20.
Marshallagia marshalli is found in the abomasums of sheep, goats and wild ruminants in tropical and sub tropical climates. In Iran among different species of Ostertagiinae that can infect sheep, M. marshalli is currently the major cause of parasitic gastroenteritis in ruminant. Having a donor animal is essential for various studies. In the present study we compared the efficacy of two different method of M. marshalli donor sheep production. In the first method, M. marshalli donor sheep was produced by transplanting of adult forms of this worm into abomasum of a lamb (6 months of age) through a surgically established cannula. In the second method, 5,000 infective larvae (L3) from the female M. marshalli culture were given to a worm-free lamb of 6 months age through a stomach tube. After 3 days of transplanting, few eggs of M. marshalli appeared in the cannulated lamb's faeces. The number of eggs per gram of faeces (EPG) increased in the following days. The average number of EPG reached up to 23.5 ± 11.26 per day in 2 months. In larval infected lamb by day 21 post infection the eggs were appeared in faeces. The average number of EPG reached up 53.5 ± 42.5 per day in 2 months. In comparison between cannulation and larval infected, the number of eggs laid by worms transplanted in cannulated lamb was less than that of larval infected lamb. However, the abomasal cannulation method seems more preferable due to some advantages such as defined number of worms transplanted into abomasums, rapid access to the eggs and their culture.
马氏马歇尔线虫寄生于热带和亚热带气候地区绵羊、山羊及野生反刍动物的皱胃中。在伊朗，能感染绵羊的各种奥斯特他线虫属物种中，马氏马歇尔线虫目前是反刍动物寄生性肠胃炎的主要病因。拥有供体动物对各种研究至关重要。在本研究中，我们比较了两种不同方法生产马氏马歇尔线虫供体绵羊的效果。第一种方法是，通过手术建立的套管将该线虫的成虫移植到一只6月龄羔羊的皱胃中，以此来生产马氏马歇尔线虫供体绵羊。第二种方法是，通过胃管将来自雌性马氏马歇尔线虫培养物的5000条感染性幼虫（L3）给予一只6月龄的无虫羔羊。移植3天后，插管羔羊的粪便中出现了少量马氏马歇尔线虫卵。随后几天，每克粪便中的虫卵数（EPG）增加。2个月内，EPG的日均平均数达到23.5±11.26。在感染幼虫的羔羊中，感染后第21天粪便中出现虫卵。2个月内，EPG的日均平均数达到53.5±42.5。在插管法和幼虫感染法的比较中，插管羔羊中移植的线虫所产的卵数少于幼虫感染的羔羊。然而，由于一些优势，如移植到皱胃中的线虫数量明确、能快速获取虫卵及其培养物，皱胃插管法似乎更可取。
