Pfeffer A, Douch P G, Shaw R J, Gatehouse T K, Rabel B, Green R S, Shirer C L, Jonas W E, Bisset S
Wallaceville Animal Research Centre, AgResearch, Upper Hutt, New Zealand.
Int J Parasitol. 1996 Jul;26(7):765-73. doi: 10.1016/0020-7519(96)00052-5.
Abomasal cannulae were surgically placed in 7 2-year-old New Zealand Romney sheep which had been maintained parasite-free from birth. Four of these sheep were randomly selected and dosed orally with 10,000 infective Trichostrongylus axei larvae per week for 8 weeks, while the remaining 3 sheep served as uninfected controls. Abomasal biopsy, blood and faecal samples were obtained from all sheep at regular intervals from 5 days before and until 58 days after the first infection. The sheep were then killed, worm burdens assessed and abomasal and small intestinal samples collected Faecal egg counts of all 4 dosed sheep were low and only one (No. 701) had a substantial worm burden (8400) post mortem. Overall, levels of mucosal mast cells/globule leukocytes, eosinophils, T19+ cells and larval migration inhibitory activity increased significantly in the abomasal mucosa of the dosed sheep compared to the controls. The CD4+:CD8+ cell ratio in the abomasal mucosa of the dosed sheep also increased compared to that of the controls (P = 0.06). In blood, T. axei-specific antibody (total and IgG1) and eosinophil numbers increased significantly in the dosed sheep. Mucosal cells staining for IgE (IgE+), and blood and mucosal eosinophils showed the earliest substantive increases in number followed by increases in specific serum antibody levels, numbers of mucosal cells fluorescing under UV light (UVf) and T19+ cells. The difference in the IgE+ and UVf cell responses indicated that expansion of globule leukocyte numbers lagged behind that of mucosal mast cells. The results supported the concept of CD4+ T cell help in the abomasal mucosa and defined the sequential expression of components of the immunological responses potentially mediating resistance to T. axei. In sheep No. 701, persistence of adult worms was associated with lower mucosal IgE+ cell and eosinophil responses compared with the other dosed sheep.
对7只2岁的新西兰罗姆尼羊进行手术植入皱胃套管，这些羊自出生起就未感染寄生虫。从这7只羊中随机选取4只，每周口服10000条感染性的奥氏毛圆线虫幼虫，持续8周，而其余3只羊作为未感染的对照。在首次感染前5天直至感染后58天，定期从所有羊身上采集皱胃活检组织、血液和粪便样本。然后处死这些羊，评估虫负荷，并采集皱胃和小肠样本。所有4只给药羊的粪便虫卵计数都很低，只有一只（701号）在死后有大量虫负荷（8400条）。总体而言，与对照组相比，给药羊皱胃黏膜中的黏膜肥大细胞/球样白细胞、嗜酸性粒细胞、T19 +细胞水平以及幼虫迁移抑制活性显著增加。给药羊皱胃黏膜中的CD4 +:CD8 +细胞比率也高于对照组（P = 0.06）。在血液中，给药羊体内奥氏毛圆线虫特异性抗体（总抗体和IgG1）以及嗜酸性粒细胞数量显著增加。对IgE染色的黏膜细胞（IgE +）、血液和黏膜嗜酸性粒细胞数量最早出现实质性增加，随后是特异性血清抗体水平、在紫外线下荧光的黏膜细胞数量（UVf）和T19 +细胞增加。IgE +和UVf细胞反应的差异表明球样白细胞数量的增加落后于黏膜肥大细胞。这些结果支持了CD4 + T细胞在皱胃黏膜中发挥辅助作用的概念，并确定了可能介导对奥氏毛圆线虫抗性的免疫反应成分的顺序表达。在701号羊中，与其他给药羊相比，成虫的持续存在与较低的黏膜IgE +细胞和嗜酸性粒细胞反应有关。
