Javanbakht Mohammad H, Sadria Reza, Djalali Mahmoud, Derakhshanian Hoda, Hosseinzadeh Payam, Zarei Mahnaz, Azizi Gholamreza, Sedaghat Reza, Mirshafiey Abbas
Nefrologia. 2014;34(4):483-90. doi: 10.3265/Nefrologia.pre2014.Jun.12051.
Nephrotic syndrome is a chronic disease especially common in the childhood and adolescence. Reactive oxygen species (ROS) and free radicals have significant role in the pathogenesis of nephrotic syndrome. The aim of this study was to evaluate the effect of soy protein and genistein (main isoflavone of soybean) on renal antioxidant status of nephrotic rats.
This study was done for 8 weeks on 40 adult male Sprague-Dawley rats divided into four groups of 10 rats each. Study groups included: 1-Control, 2-Nephrotic syndrome, 3-Nephrotic syndrome+soy protein diet and 4-Nephrotic syndrome+soy protein diet+genistein. Urine protein and urine creatinine were measured. After homogenization of kidney, total antioxidant capacities (TAC), activities of catalase enzyme, the concentration of malondialdehydes (MDA) and carbolynated proteins were determined spectrophotometrically. Pathological examination was done on kidneys with light microscope. Cell viability was evaluated with MTT assay on WEHI-164 fibro sarcoma cell line. The MMP2 enzyme activity was evaluated in different concentrations of genistein.
Total antioxidant capacity was significantly increased in soy genistein. Catalase activity was significantly increased in soy and soy genistein groups. Protein carbonyl and MDA were significantly lower in soy and soy genistein groups. The scores of pathological examination showed significant improvement in soy and soy genistein groups. Genistein decreased the proliferation of the WEHI-164 fibrosarcoma cell line.
It seems that soy protein decreases kidney damages in nephrotic syndrome. Adding genistein to soy protein causes improvements in antioxidant status of kidney tissue. Genistein decreases proliferation of cell.
肾病综合征是一种慢性疾病，在儿童和青少年中尤为常见。活性氧（ROS）和自由基在肾病综合征的发病机制中起重要作用。本研究旨在评估大豆蛋白和染料木黄酮（大豆的主要异黄酮）对肾病大鼠肾脏抗氧化状态的影响。
本研究对40只成年雄性Sprague-Dawley大鼠进行了为期8周的实验，将其分为四组，每组10只。研究组包括：1-对照组，2-肾病综合征组，3-肾病综合征+大豆蛋白饮食组，4-肾病综合征+大豆蛋白饮食+染料木黄酮组。测量尿蛋白和尿肌酐。肾脏匀浆后，用分光光度法测定总抗氧化能力（TAC）、过氧化氢酶活性、丙二醛（MDA）浓度和羰基化蛋白。用光学显微镜对肾脏进行病理检查。用MTT法在WEHI-164纤维肉瘤细胞系上评估细胞活力。在不同浓度的染料木黄酮中评估MMP2酶活性。
大豆染料木黄酮组的总抗氧化能力显著增加。大豆组和大豆染料木黄酮组的过氧化氢酶活性显著增加。大豆组和大豆染料木黄酮组的蛋白羰基和MDA显著降低。病理检查评分显示大豆组和大豆染料木黄酮组有显著改善。染料木黄酮降低了WEHI-164纤维肉瘤细胞系的增殖。
大豆蛋白似乎能减轻肾病综合征中的肾脏损伤。在大豆蛋白中添加染料木黄酮可改善肾脏组织的抗氧化状态。染料木黄酮可降低细胞增殖。
