Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.
Branch for Northwest & West Region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran.
Mol Biol Rep. 2020 Jan;47(1):655-669. doi: 10.1007/s11033-019-05173-z. Epub 2019 Nov 9.
Tall fescue is a perennial cool-season grass with economic importance especially in temperate regions of the northern hemisphere. This study was done to assess the genetic diversity and population structure of 90 tall fescue populations and cultivars using ISSR and EST-SSR markers in order to categorize valuable populations for breeding programs and to construct the core collection of tall fescue collection in Iran. The 10 EST-SSR primer pairs amplified 92 alleles. The allele numbers varied from 4 to 13 alleles per locus with an average of 9.2 alleles, of which 84 (90.6%) were polymorphic with an average of 8.4 polymorphic bands per primer. The 39 ISSR primers totally produced 387 scorable bands, of which 335 (86.6%) were polymorphic with an average of 8.6 polymorphic bands per primer. The amplified markers by ISSR primers varied from 6 to 14 markers per primer with an average of 9.92 markers per primer. The 90 tall fescue populations using both EST-SSR and ISSR data were classified into two clusters by UPGMA method that was coincide with PCA and structure analysis results. The turf-type and forage-type populations were clearly separated. Based on the results, the Iranian populations provide a valuable and novel germplasm to employ in tall fescue varietal improvement programs for both forage and turf-type applications. This progression is an important step to introduce this collection for development of a core collection of tall fescue germplasm in Iran.
高羊茅是一种多年生冷季型草，在北半球温带地区具有重要的经济意义。本研究采用 ISSR 和 EST-SSR 标记评估了 90 个高羊茅群体和品种的遗传多样性和群体结构，以便为育种计划分类有价值的群体，并构建伊朗高羊茅群体的核心收集。10 对 EST-SSR 引物共扩增出 92 个等位基因。每个位点的等位基因数从 4 到 13 个不等，平均为 9.2 个，其中 84 个（90.6%）为多态性，平均每个引物有 8.4 个多态性条带。39 对 ISSR 引物共产生 387 个可评分条带，其中 335 个（86.6%）为多态性，平均每个引物有 8.6 个多态性条带。ISSR 引物扩增的标记数从每个引物 6 到 14 个不等，平均每个引物 9.92 个标记。利用 EST-SSR 和 ISSR 数据，90 个高羊茅群体通过 UPGMA 法分为两个聚类，这与 PCA 和结构分析结果一致。草坪型和饲草型群体明显分开。基于这些结果，伊朗群体为高羊茅品种改良计划提供了有价值的新种质资源，适用于饲草和草坪型应用。这一进展是为伊朗高羊茅种质资源核心收集的发展引入该收集的重要步骤。
