Kaur Gurpreet, Singh Deepinder, Brar Vivekjot
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India.
Int J Biol Macromol. 2014 Sep;70:408-19. doi: 10.1016/j.ijbiomac.2014.07.015. Epub 2014 Jul 15.
In the present investigation, polysaccharide from the Okra fruits (Hibiscus esculentus) was extracted, characterized and explored for its mucoadhesive potential. Mucoadhesive films of okra polymer (OP) were prepared by solvent casting method based on 3(2) factorial design. For these studies, OP (2.0%, 2.5%, 3.0%, w/v) and glycerol (plasticizer) (0.25%, 0.50%, 0.75%, v/v) were taken as independent variables while tensile strength, mucoadhesive strength, contact angle, swelling index and residence time as dependent variables. The developed films were evaluated for their physicochemical, mechanical and electrical properties. The formulated films were found to be smooth, flexible, and displayed adequate mucoadhesive and tensile strength. Their near neutral pH and negative hemolytic studies indicated their non-irritability and biocompatible nature with biological tissues. The formulation comprising of 3% OP and 0.5% glycerol (F8) was found to exhibit optimum mechanical properties. Further, optimized film was loaded with zolmitriptan (model drug) to determine its drug release profiles. In vitro and ex vivo drug release studies demonstrated a controlled release of zolmitriptan over a period of 8h in simulated salivary fluid (SSF) pH 6.8, with the correlation coefficient values indicating its non-Fickian kinetics. Thus, OP can be used as a promising biomaterial for controlled drug delivery.
在本研究中，对秋葵果实（黄秋葵）中的多糖进行了提取、表征，并探究了其黏膜黏附潜力。基于3（2）析因设计，采用溶剂浇铸法制备了秋葵聚合物（OP）黏膜黏附膜。在这些研究中，将OP（2.0%、2.5%、3.0%，w/v）和甘油（增塑剂）（0.25%、0.50%、0.75%，v/v）作为自变量，而将拉伸强度、黏膜黏附强度、接触角、溶胀指数和滞留时间作为因变量。对所制备的膜的物理化学、机械和电学性质进行了评估。所制备的膜被发现是光滑、柔韧的，并且具有足够的黏膜黏附性和拉伸强度。它们接近中性的pH值和阴性溶血研究表明它们对生物组织无刺激性且具有生物相容性。发现由3% OP和0.5%甘油组成的配方（F8）表现出最佳的机械性能。此外，将优化后的膜负载佐米曲普坦（模型药物）以确定其药物释放曲线。体外和离体药物释放研究表明，在pH 6.8的模拟唾液（SSF）中，佐米曲普坦在8小时内实现了控释，相关系数值表明其为非菲克动力学。因此，OP可作为一种有前景的生物材料用于控释给药。
