Pan Hongbo, Li Lifang, Lin Xiaofeng, Li Jiqiu, Al-Farraj Saleh A, Al-Rasheid Khaled A
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
J Eukaryot Microbiol. 2014 Nov-Dec;61(6):644-54. doi: 10.1111/jeu.12146. Epub 2014 Aug 21.
Two new and one problematic species of pleurostomatids, Amphileptus dragescoi sp. n., A. wilberti sp. n., and A. marinus from coastal areas of the South China Sea, are described based on observations of live and protargol-impregnated specimens. Amphileptus dragescoi is different from its congeners by the presence of an apical group of extrusomes and the possession of 12-15 right and five left somatic kineties, two macronuclear nodules, and a single terminally positioned contractile vacuole. Amphileptus wilberti is diagnosed by oval or pyriform body, 15-19 right and seven or eight left somatic kineties, extrusomes arranged only in anterior portion of oral slit, usually three ventrally located contractile vacuoles, and two macronuclear nodules. Amphileptus marinus (Kahl, 1931) Song et al., 2004 is redescribed and its diagnosis is improved. One isolate which was misidentified as A. marinus by Song et al. (2004) is believed to represent an unknown form, named here as Amphileptus songi sp. n. Phylogenetic analyses of the SSU rDNA sequences indicate that the genus Amphileptus is paraphyletic, but its monophyly is not rejected by statistical tree topology tests.
基于对活体和经原银染标本的观察，描述了采自中国南海沿岸地区的3种侧口虫，即德拉热侧口虫（Amphileptus dragescoi sp. n.）、威尔伯侧口虫（A. wilberti sp. n.）和海生侧口虫（A. marinus），其中2种为新物种，1种存在问题。德拉热侧口虫与其同属物种的区别在于，其顶端有一组射出体，有12 - 15条右侧和5条左侧体动基列、两个大核结节以及一个位于末端的收缩泡。威尔伯侧口虫的诊断特征为：虫体呈椭圆形或梨形，有15 - 19条右侧和7或8条左侧体动基列，射出体仅排列在口沟前部，通常有3个位于腹面的收缩泡，以及两个大核结节。对海生侧口虫（Amphileptus marinus (Kahl, 1931) Song et al., 2004）进行了重新描述并改进了其诊断特征。有一个分离株被Song等人（2004年）误鉴定为海生侧口虫，现认为它代表一种未知形态，在此命名为宋氏侧口虫（Amphileptus songi sp. n.）。对小亚基核糖体DNA（SSU rDNA）序列的系统发育分析表明，侧口虫属是并系的，但统计树拓扑检验并未否定其单系性。
