Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai 201306, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Eur J Protistol. 2022 Feb;82:125854. doi: 10.1016/j.ejop.2021.125854. Epub 2021 Dec 3.
The morphology and phylogeny of two new species of pleurostomatid ciliates, Amphilepus weishanensis sp. nov. and A. parapleurosigma sp. nov., collected from Lake Weishan in northern China, were studied using live observation, protargol staining and phylogenetic analyses based on small subunit ribosomal DNA (SSU rDNA) sequence data. In both species, extrusomes form an apical group and contractile vacuoles are distributed along both the dorsal and the ventral margin. Amphileptus weishanensis sp. nov. is characterized by its body size (560-780 × 60-100 μm in vivo) and the possession of five left and 56-61 right somatic kineties, filiform extrusomes and 3-9 macronuclear nodules. Amphileptus parapleurosigma sp. nov. is characterized by possessing 4-6 left and 19-24 right somatic kineties and clavate extrusomes. The SSU rDNA sequences differ among ten similar species by 3-60 bp. Phylogenetic analyses based on the SSU rDNA sequences indicate that the family Amphileptidae is monophyletic while Amphileptus is paraphyletic with Pseudoamphileptus nesting within this genus.
两种新的 Pleurostomatid 纤毛虫，即来自中国北方微山湖的新种 Amphilepus weishanensis 和 A. parapleurosigma 的形态和系统发育，通过活体观察、蛋白银染色和基于小亚基核糖体 DNA（SSU rDNA）序列数据的系统发育分析进行了研究。在这两个物种中，挤出体形成一个顶端群，收缩泡沿背腹缘分布。Amphileptus weishanensis sp. nov. 的特征是其体型（活体大小为 560-780×60-100μm）和 5 个左侧和 56-61 个右侧体纤毛带，丝状挤出体和 3-9 个大核小结。Amphileptus parapleurosigma sp. nov. 的特征是具有 4-6 个左侧和 19-24 个右侧体纤毛带和棍棒状挤出体。SSU rDNA 序列在十个相似物种之间存在 3-60bp 的差异。基于 SSU rDNA 序列的系统发育分析表明，Amphileptidae 科是单系的，而 Amphileptus 是并系的，Pseudoamphileptus 嵌套在这个属内。
