Davaatseren Munkhtugs, Hwang Jin-Taek, Park Jae Ho, Kim Myung-Sunny, Wang Shuaiyu, Sung Mi Jeong
Research Division Emerging Innovative Technology, Korea Food Research Institute, Songnam, Keongki, Republic of Korea; Department of Bioresources and Food Science, Konkuk University, Seoul, Republic of Korea.
Research Division Emerging Innovative Technology, Korea Food Research Institute, Songnam, Keongki, Republic of Korea.
PLoS One. 2014 Jul 22;9(7):e102975. doi: 10.1371/journal.pone.0102975. eCollection 2014.
Allyl isothiocyanate (AITC) is a phytochemical found in cruciferous vegetables that has known chemopreventive and chemotherapeutic activities. Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflammation in a mouse model of colitis. Experimental colitis was induced in mice by administering 3% dextran sulfate sodium via drinking water. To monitor the activity of AITC in this model, we measured body weight, disease activity indices, histopathological scores, microvascular density, myeloperoxidase activity, F4/80 staining, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and vascular endothelial growth factor (VEGF)-A/VEGF receptor 2 (VEGFR2) expression in the mice. We found that AITC-treated mice showed less weight loss, fewer clinical signs of colitis, and longer colons than vehicle-treated mice. AITC treatment also significantly lessened the disruption of colonic architecture that is normally associated with colitis and repressed the microvascularization response. Further, AITC treatment reduced both leukocyte recruitment and macrophage infiltration into the inflamed colon, and the mechanism these activities involved repressing iNOS and COX-2 expression. Finally, AITC attenuated the expression of VEGF-A and VEGFR2. Thus, AITC may have potential application in treating conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.
异硫氰酸烯丙酯（AITC）是一种存在于十字花科蔬菜中的植物化学物质，已知具有化学预防和化学治疗活性。迄今为止，AITC的抗血管生成活性尚未在体内研究中报道。在此，我们研究了AITC对结肠炎小鼠模型中血管生成和炎症的影响。通过给小鼠饮用3%的葡聚糖硫酸钠诱导实验性结肠炎。为了监测AITC在该模型中的活性，我们测量了小鼠的体重、疾病活动指数、组织病理学评分、微血管密度、髓过氧化物酶活性、F4/80染色、诱导型一氧化氮合酶（iNOS）表达、环氧合酶-2（COX-2）表达以及血管内皮生长因子（VEGF）-A/血管内皮生长因子受体2（VEGFR2）表达。我们发现，与载体处理的小鼠相比，AITC处理的小鼠体重减轻更少，结肠炎的临床症状更少，结肠更长。AITC处理还显著减轻了通常与结肠炎相关的结肠结构破坏，并抑制了微血管化反应。此外，AITC处理减少了白细胞募集和巨噬细胞向炎症结肠的浸润，这些活动的机制包括抑制iNOS和COX-2表达。最后，AITC减弱了VEGF-A和VEGFR2的表达。因此，AITC在治疗以炎症驱动的血管生成和粘膜炎症为特征的疾病方面可能具有潜在应用。
