Research Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of Korea.
Mediators Inflamm. 2013;2013:982383. doi: 10.1155/2013/982383. Epub 2013 May 16.
Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium-(DSS-) induced mouse colitis model. Experimental colitis was induced in male C57BL/6 mice by administering 3% DSS. Disease activity index (DAI), histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg/kg body weight per day or 3% DSS with γ-PGA at 200 mg/kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.
聚-γ-谷氨酸（γ-PGA）是一种天然分泌自各种芽孢杆菌菌株的物质，具有抗炎活性。在炎症性肠病（IBD）中，炎症是由血管生成促进和维持的；然而，γ-PGA 在这种情况下的作用尚不清楚。因此，我们在葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型中评估了 γ-PGA 对血管生成和炎症的影响。雄性 C57BL/6 小鼠通过给予 3% DSS 来诱导实验性结肠炎。在对照组小鼠、DSS 处理组小鼠以及每天给予 50mg/kg 体重γ-PGA 的 3% DSS 组小鼠或每天给予 200mg/kg 体重γ-PGA 的 3% DSS 组小鼠中比较疾病活动指数（DAI）、组织病理学评分、微血管密度、髓过氧化物酶活性以及 VEGF-A 和 VEGFR2 的表达。我们发现 γ-PGA 显著减轻了体重减轻、DAI 和结肠缩短。γ-PGA 还显著减轻了组织学损伤的证据。此外，γ-PGA 显著减弱了 DSS 诱导的血管密度。此外，γ-PGA 减弱了 DSS 诱导的 VEGF-A 及其受体 VEGFR2 的表达。此外，γ-PGA 治疗导致炎症性结肠炎中白细胞募集减少。因此，我们的结果表明，γ-PGA 在以炎症驱动的血管生成和黏膜炎症为特征的情况下具有潜在的应用价值。
