Silveira Júlia Angélica Gonçalves da, Rabelo Elida Mara Leite, Lima Paula Cristina Senra, Chaves Bárbara Neves, Ribeiro Múcio Flávio Barbosa
Departamento de Parasitologia, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
Departamento de Biologia Geral, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
Rev Bras Parasitol Vet. 2014 Apr-Jun;23(2):206-15. doi: 10.1590/s1984-29612014035.
Tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. The aim of the present study was the post-mortem detection of hemoparasites in free-living Mazama gouazoubira from Minas Gerais state, Brazil. The deer samples consisted of free-living M. gouazoubira (n = 9) individuals that died after capture. Necropsy examinations of the carcasses were performed to search for macroscopic alterations. Organ samples were collected for subsequent imprint slides, and nested PCR assays were performed to detect hemoparasite species. Imprint slide assays from four deer showed erythrocytes infected with Piroplasmida small trophozoites, and A. marginale corpuscles were observed in erythrocytes from two animals. A. marginale and trophozoite co-infections occurred in two deer. A nested PCR analysis of the organs showed that six of the nine samples were positive for Theileria sp., five were positive for A. phagocytophilum and three were positive for A. marginale, with co-infection occurring in four deer. The results of the present study demonstrate that post-mortem diagnostics using imprint slides and molecular assays are an effective method for detecting hemoparasites in organs.
蜱传感染可给野生反刍动物带来严重的健康问题，其中一些感染因子可被视为人畜共患病原。本研究的目的是对来自巴西米纳斯吉拉斯州的野生南美泽鹿进行死后血液寄生虫检测。鹿样本包括9只捕获后死亡的野生南美泽鹿个体。对鹿尸体进行尸检以寻找宏观病变。采集器官样本用于后续印片，并行巢式PCR检测以确定血液寄生虫种类。四只鹿的印片检测显示红细胞被梨形虫小滋养体感染，两只动物的红细胞中观察到边缘无形体小体。两只鹿发生了边缘无形体和滋养体混合感染。对器官进行的巢式PCR分析显示，九个样本中有六个泰勒虫属呈阳性，五个嗜吞噬细胞无形体呈阳性，三个边缘无形体呈阳性，四只鹿发生了混合感染。本研究结果表明，使用印片和分子检测进行死后诊断是检测器官中血液寄生虫的有效方法。
