一种用于禽流感病毒监测和亚型分型的完整分子诊断程序。
A complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus.
作者信息
Tseng Chun-Hsien, Tsai Hsiang-Jung, Chang Chung-Ming
机构信息
Animal Health Research Institute, Danshui, Taipei 25158, Taiwan.
Animal Health Research Institute, Danshui, Taipei 25158, Taiwan ; Graduate Institute of Veterinary Medicine, College of Bioresource and Agriculture, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
出版信息
Biomed Res Int. 2014;2014:653056. doi: 10.1155/2014/653056. Epub 2014 Jun 26.
Abstract
INTRODUCTION
The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.
METHOD
This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes.
RESULT AND CONCLUSION
This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.
摘要
引言
我们基于实时定量PCR和传统PCR开发的以下完整分子诊断程序，对禽流感监测、病毒亚型分型和病毒基因组测序有效。
方法
本研究提供了一个针对16种血凝素和9种神经氨酸酶禽流感亚型进行高效禽流感鉴定的具体且灵敏的分步程序。
结果与结论
这一诊断程序可能对全球禽流感研究的病毒学和生态学进展极为有用。
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