Li Jinling, Cardona Carol J, Xing Zheng, Woolcock Peter R
Department of Population Health and Reproduction, University of California, Surge III, One Shields Avenue, Davis, CA 95616, USA.
Arch Virol. 2008;153(10):1899-908. doi: 10.1007/s00705-008-0217-4. Epub 2008 Oct 1.
An H11N9 low-pathogenicity avian influenza virus, A/duck/WA/663/97, was isolated from a sick Mandarin duck kept in an outdoor bird exhibit. Genetic and phenotypic characterization of the virus suggested that it originated from free-flying birds, a concept supported by genetic similarity with waterfowl isolates from the same geographic area and time period. This duck-origin virus had genetic features typical of H11 and N9 viruses, including no neuraminidase stalk deletion, no differences in putative glycosylation sites in either surface protein, and no addition of basic amino acid residues at the hemagglutinin cleavage site compared to published sequences. It replicated in both avian and mammalian cells in vitro, and experimentally challenged chickens developed mild acute upper respiratory lesions but no clinical signs of disease. It elicited immune responses in chickens, resulting in seroconversion in all infected birds, although antibody titers remained low over the experimental period.
一种H11N9低致病性禽流感病毒，A/duck/WA/663/97，从一只饲养在室外鸟类展区的患病鸳鸯中分离出来。对该病毒的基因和表型特征分析表明，它起源于自由飞行的鸟类，这一观点得到了与同一地理区域和时间段的水禽分离株基因相似性的支持。这种源自鸭子的病毒具有H11和N9病毒的典型基因特征，包括没有神经氨酸酶茎部缺失，两种表面蛋白的假定糖基化位点没有差异，并且与已发表序列相比，血凝素裂解位点没有添加碱性氨基酸残基。它在体外的禽类和哺乳动物细胞中均能复制，经实验感染的鸡出现轻度急性上呼吸道病变，但没有疾病的临床症状。它在鸡中引发了免疫反应，导致所有感染鸟类发生血清转化，尽管在实验期间抗体滴度仍然较低。
