Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, Cidade Universitária, São Paulo, SP, CEP 05508-000, Brazil.
J Ind Microbiol Biotechnol. 2014 Sep;41(9):1353-63. doi: 10.1007/s10295-014-1485-5. Epub 2014 Jul 25.
Burkholderia sp. F24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (P3HB). Using non-detoxified hydrolysate, Burkholderia sp. F24 reached a cell dry weight (CDW) of 6.8 g L(-1), containing 48 % of P3HB and exhibited a volumetric productivity (PP3HB) of 0.10 g L(-1) h(-1). Poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (P3HB-co-3HV) were produced using xylose and levulinic acid (LA) as carbon sources. In shake flask cultures, the 3HV content in the copolymer increased from 9 to 43 mol% by adding LA from 1.0 to 5.0 g L(-1). In high cell density cultivation using concentrated hemicellulosic hydrolysate F24 reached 25.04 g L(-1) of CDW containing 49 % of P3HB and PP3HB of 0.28 g L(-1 )h(-1). Based on these findings, second-generation ethanol and bioplastics from sugarcane bagasse is proposed.
最初从土壤中分离出的伯克霍尔德氏菌 F24 能够在木糖上生长，并去除半纤维素水解物中存在的有机抑制剂，同时生产聚 3-羟基丁酸酯 (P3HB)。使用未经解毒的水解物，伯克霍尔德氏菌 F24 达到了 6.8 g/L 的细胞干重 (CDW)，其中含有 48%的 P3HB，表现出 0.10 g/L/h 的体积生产率 (PP3HB)。使用木糖和乙酰丙酸 (LA) 作为碳源生产聚 3-羟基丁酸酯-共-3-羟基戊酸共聚物 (P3HB-co-3HV)。在摇瓶培养中，通过从 1.0 到 5.0 g/L 添加 LA，共聚物中的 3HV 含量从 9 增加到 43 mol%。在使用浓缩半纤维素水解物的高密度细胞培养中，F24 达到了 25.04 g/L 的 CDW，其中含有 49%的 P3HB 和 0.28 g/L/h 的 PP3HB。基于这些发现，提出了第二代乙醇和甘蔗渣生物塑料。
