用于研究造血发育的转基因小鼠荧光报告基因系的构建
Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.
作者信息
Vacaru Andrei M, Vitale Joseph, Nieves Johnathan, Baron Margaret H
机构信息
Department of Medicine, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, 1079, New York, NY, 10029-6574, USA.
出版信息
Methods Mol Biol. 2014;1194:289-312. doi: 10.1007/978-1-4939-1215-5_16.
Abstract
During the development of the hematopoietic system, at least eight distinct lineages are generated in the mouse embryo. Transgenic mice expressing fluorescent proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to multipotent progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene and survey available fluorescent probes and methods for confirming and analyzing transgene expression in the hematopoietic systems of the embryo, fetus, and postnatal/adult animal.
摘要
在造血系统发育过程中，小鼠胚胎中至少会产生八个不同的谱系。从造血干细胞到多能祖细胞，再到每种最终分化细胞类型，在造血层级不同阶段表达荧光蛋白的转基因小鼠，为标记、追踪和分离这些细胞提供了有价值的工具。在本章中，我们将讨论设计转基因时的一般注意事项，并概述可用的荧光探针以及用于确认和分析胚胎、胎儿及出生后/成年动物造血系统中转基因表达的方法。
相似文献
1
Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.
Methods Mol Biol. 2014;1194:289-312. doi: 10.1007/978-1-4939-1215-5_16.
2
Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.
Methods Mol Biol. 2021;2224:153-182. doi: 10.1007/978-1-0716-1008-4_12.
3
Hoxb4-YFP reporter mouse model: a novel tool for tracking HSC development and studying the role of Hoxb4 in hematopoiesis.
Blood. 2011 Mar 31;117(13):3521-8. doi: 10.1182/blood-2009-12-253989. Epub 2011 Jan 28.
4
Fluorescent genetic barcoding for cellular multiplex analyses.
Exp Hematol. 2018 Nov;67:10-17. doi: 10.1016/j.exphem.2018.08.001. Epub 2018 Aug 8.
5
Use of transgenic fluorescent reporter mouse lines to monitor hematopoietic and erythroid development during embryogenesis.
Methods Enzymol. 2010;476:403-27. doi: 10.1016/S0076-6879(10)76022-5.
6
Abcg2 expression marks tissue-specific stem cells in multiple organs in a mouse progeny tracking model.
Stem Cells. 2012 Feb;30(2):210-21. doi: 10.1002/stem.1002.
7
Sertoli cell behaviors in developing testis cords and postnatal seminiferous tubules of the mouse.
Biol Reprod. 2011 Feb;84(2):342-50. doi: 10.1095/biolreprod.110.086900. Epub 2010 Oct 13.
8
Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method.
Biochem Biophys Res Commun. 2013 Jun 14;435(4):586-91. doi: 10.1016/j.bbrc.2013.05.017. Epub 2013 May 17.
9
A dual-fluorescence reporter in the Eomes locus for live imaging and medium-term lineage tracing.
Genesis. 2017 Aug;55(8). doi: 10.1002/dvg.23043. Epub 2017 Jul 14.
10
Direct isolation of committed neuronal progenitor cells from transgenic mice coexpressing spectrally distinct fluorescent proteins regulated by stage-specific neural promoters.
J Neurosci Res. 2001 Aug 1;65(3):220-7. doi: 10.1002/jnr.1145.
引用本文的文献
1
A User-Friendly Approach for Routine Histopathological and Morphometric Analysis of Skeletal Muscle Using CellProfiler Software.
Diagnostics (Basel). 2022 Feb 22;12(3):561. doi: 10.3390/diagnostics12030561.
2
Expression of a membrane-targeted fluorescent reporter disrupts auditory hair cell mechanoelectrical transduction and causes profound deafness.
Hear Res. 2021 May;404:108212. doi: 10.1016/j.heares.2021.108212. Epub 2021 Feb 18.
3
Environmental exposures, stem cells, and cancer.
Pharmacol Ther. 2019 Dec;204:107398. doi: 10.1016/j.pharmthera.2019.107398. Epub 2019 Jul 31.
4
Progress in brain cannabinoid CB receptor research: From genes to behavior.
Neurosci Biobehav Rev. 2019 Mar;98:208-220. doi: 10.1016/j.neubiorev.2018.12.026. Epub 2019 Jan 3.
5
Activation of the vitamin D receptor transcription factor stimulates the growth of definitive erythroid progenitors.
Blood Adv. 2018 Jun 12;2(11):1207-1219. doi: 10.1182/bloodadvances.2018017533.
6
A Naturally Fluorescent Mgp Transgenic Mouse for Angiogenesis and Glaucoma Longitudinal Studies.
Invest Ophthalmol Vis Sci. 2018 Feb 1;59(2):746-756. doi: 10.1167/iovs.17-22992.
7
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow.
J Vis Exp. 2016 Jul 28(113):54205. doi: 10.3791/54205.
本文引用的文献
1
A dual reporter mouse model of the human β-globin locus: applications and limitations.
PLoS One. 2012;7(12):e51272. doi: 10.1371/journal.pone.0051272. Epub 2012 Dec 14.
2
Mapping differentiation pathways from hematopoietic stem cells using Flk2/Flt3 lineage tracing.
Cell Cycle. 2012 Sep 1;11(17):3180-8. doi: 10.4161/cc.21279. Epub 2012 Aug 16.
3
Dicer is selectively important for the earliest stages of erythroid development.
Blood. 2012 Sep 20;120(12):2412-6. doi: 10.1182/blood-2011-10-383653. Epub 2012 Aug 6.
4
ETV2 expression marks blood and endothelium precursors, including hemogenic endothelium, at the onset of blood development.
Dev Dyn. 2012 Sep;241(9):1454-64. doi: 10.1002/dvdy.23825. Epub 2012 Jul 16.
5
Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.
Stem Cells. 2012 Aug;30(8):1611-23. doi: 10.1002/stem.1131.
6
The embryonic origins of erythropoiesis in mammals.
Blood. 2012 May 24;119(21):4828-37. doi: 10.1182/blood-2012-01-153486. Epub 2012 Feb 15.
7
c-Kit-mediated functional positioning of stem cells to their niches is essential for maintenance and regeneration of adult hematopoiesis.
PLoS One. 2011;6(10):e26918. doi: 10.1371/journal.pone.0026918. Epub 2011 Oct 26.
8
The miR-144/451eGFP allele, a novel tool for resolving the erythroid potential of hematopoietic precursors.
Blood. 2011 Sep 15;118(11):2988-92. doi: 10.1182/blood-2011-04-350728. Epub 2011 Jul 25.
9
Hoxb4-YFP reporter mouse model: a novel tool for tracking HSC development and studying the role of Hoxb4 in hematopoiesis.
Blood. 2011 Mar 31;117(13):3521-8. doi: 10.1182/blood-2009-12-253989. Epub 2011 Jan 28.
10
Imaging mouse development with confocal time-lapse microscopy.
Methods Enzymol. 2010;476:351-77. doi: 10.1016/S0076-6879(10)76020-1.
Zotero 插件