Department of Environmental Engineering, Faculty of Engineering, Khon Kaen University, 123 Mittapap Road, Muang District, Khon Kaen, 40002, Thailand,
Environ Sci Pollut Res Int. 2015 Jan;22(1):320-8. doi: 10.1007/s11356-014-3354-1. Epub 2014 Jul 29.
Profenofos (PF) is one of the heavily used organophosphorus pesticides (OPPs) of which its contamination is ubiquitous in an agricultural area. This study aims to acquire and characterize PF-degrading bacterial cultures from contaminated soil. OPP degradation by the novel isolates was then investigated. The experiment was performed at the initial PF concentration of 20 mg/L. The result showed that the enriched consortium comprised three predominant PF-degrading strains designated as PF1, PF2, and PF3. The isolates (PF1, PF2, and PF3) were characterized as Pseudomonas plecoglossicida, Pseudomonas aeruginosa, and P. aeruginosa, respectively. A consortium and all isolates could utilize PF as a sole carbon source with PF removal of more than 90% via a hydrolysis process. The bacterial growth and PF degradation rates followed the first-order kinetic reaction with the rates of 0.4 to 2.7/h and 0.15 to 1.96/h, respectively. Additional carbon supplement deteriorated PF biodegradation. The enriched cultures were also capable for degrading chlorpyrifos and dicrotophos pesticides (33-73% removal). The results indicated that the consortium and isolates are efficient for PF and other OPP degradation and have potential for PF remediation.
丙溴磷（PF）是一种广泛使用的有机磷农药（OPPs），其在农业区的污染无处不在。本研究旨在从污染土壤中获得并鉴定能够降解 PF 的细菌培养物。然后研究了新型分离物对 OPP 的降解作用。实验在 PF 的初始浓度为 20mg/L 时进行。结果表明，富集的共生物群由三种主要的 PF 降解菌株组成，分别命名为 PF1、PF2 和 PF3。这些分离物（PF1、PF2 和 PF3）分别被鉴定为恶臭假单胞菌、铜绿假单胞菌和铜绿假单胞菌。共生物群和所有分离物都可以将 PF 作为唯一的碳源利用，通过水解过程去除超过 90%的 PF。细菌生长和 PF 降解速率遵循一级动力学反应，速率分别为 0.4 至 2.7/h 和 0.15 至 1.96/h。额外的碳补充会恶化 PF 的生物降解。富集培养物也能够降解氯吡硫磷和敌敌畏农药（去除率为 33-73%）。结果表明，该共生物群和分离物对 PF 和其他 OPP 的降解具有高效性，并有修复 PF 的潜力。
